Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia.
Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia.
复制标题
蛋白酶体依赖性蛋白质降解在海兔长期操作记忆中的作用。
DOI：
10.1101/lm.043794.116
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Krishnan,HariniC
中科院分区：
文献类型：
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作者：
Lyons,LisaC;Gardner,JacobS;Gandour,CatherineE;Krishnan,HariniC
我们使用操作型学习范式研究了蛋白质降解在白杨中间记忆(ITM)和长时记忆(LTM)中的作用。蛋白酶体抑制剂MG-132抑制LTM的诱导和分子固结，但对ITM无影响。值得注意的是，通过抑制蛋白质合成来维持稳定的蛋白质水平，结合蛋白酶体的抑制，可以形成强大的24小时LTM。我们的研究表明，蛋白酶体活动在促进LTM基因转录方面发挥了主要作用，并提出了突触机制足以维持24小时记忆的可能性。
We investigated the in vivo role of protein degradation during intermediate (ITM) and long-term memory (LTM) inAplysiausing an operant learning paradigm. The proteasome inhibitor MG-132 inhibited the induction and molecular consolidation of LTM with no effect on ITM. Remarkably, maintenance of steady-state protein levels through inhibition of protein synthesis using either anisomycin or rapamycin in conjunction with proteasome inhibition permitted the formation of robust 24 h LTM. Our studies suggest a primary role for proteasomal activity in facilitation of gene transcription for LTM and raise the possibility that synaptic mechanisms are sufficient to sustain 24 h memory.