Burn enhances toll-like receptor induced responses by circulating leukocytes.
Burn enhances toll-like receptor induced responses by circulating leukocytes.
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发表时间：
2012-04
影响因子：
0.1
通讯作者：
M. Schwacha;Qiong Zhang;Meenakshi Rani;T. Craig;Richard F. Oppeltz
中科院分区：
文献类型：
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作者：
M. Schwacha;Qiong Zhang;Meenakshi Rani;T. Craig;Richard F. Oppeltz
烧伤和 Toll 样受体 (TLR) 与先天免疫系统激活有关，但烧伤对循环白细胞 TLR 诱导的炎症反应的影响尚不清楚。为了研究这一点，对 C57BL/6 小鼠进行烧伤（3 度，25% TBSA）或假手术，并在 1-7 天后收集血液。在存在酵母聚糖（TLR-2 激动剂）或 LPS（TLR-4 激动剂）的情况下，将全血细胞悬浮液孵育 24 小时。血培养物对 TLR2 和 TLR4 介导的激活有反应，导致产生 IL-6、IL-10、IL-17、TNF-α、MIP-1α、MIP-1β、KC 和 RANTES。烧伤后 3-7 天，烧伤组中 TLR2 诱导的 KC 和 MIP-1β 产生较多，而烧伤后 TLR4 诱导的激活中 IL-6、IL-10、KC 和 MIP-1β 的产生较多。总之，循环白细胞对 TLR 诱导的激活有反应，并且 TLR 介导的炎症反应在损伤后 3-7 天增强，这些炎症介质的产生增加证明了这一点。
Burn and toll-like receptors (TLR) are associated with innate immune system activation, but the impact of burn on TLR-induced inflammation responses by circulating leukocytes is unknown. To study this, C57BL/6 mice were subjected to burn (3(rd) degree, 25% TBSA) or sham procedure and 1-7 days later blood was collected. Whole blood cell suspensions were incubated for 24 hr in the presence of zymosan (TLR-2 agonist) or LPS (TLR-4 agonist). The blood cultures were responsive to TLR2 and TLR4-mediated activation, resulting in the production of IL-6, IL-10, IL-17, TNF-α, MIP-1α, MIP-1β, KC and RANTES. TLR2-induced KC and MIP-1β production was greater in the burn group at 3-7 days post-injury, whereas IL-6, IL-10, KC and MIP-1β were greater for TLR4-induced activation after burn. In conclusion, circulating leukocytes were responsive to TLR-induced activation and TLR-mediated inflammatory responses were enhanced 3-7 days post-injury, as evidenced by increased production of these inflammatory mediators.