Intranasal Immunization with Live Recombinant Lactococcus lactis Combined with Heat-Labile Toxin B Subunit Protects Chickens from Highly Pathogenic Avian Influenza H5N1 Virus
Intranasal Immunization with Live Recombinant Lactococcus lactis Combined with Heat-Labile Toxin B Subunit Protects Chickens from Highly Pathogenic Avian Influenza H5N1 Virus
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使用活重组乳酸乳球菌结合不耐热毒素 B 亚基进行鼻内免疫可保护鸡免受高致病性禽流感 H5N1 病毒的侵害。
DOI：
10.1002/jmv.23983
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发表时间：
2015-01-01
影响因子：
12.7
通讯作者：
Zhao, Daxian
中科院分区：
文献类型：
--
作者：
Lei, Han;Peng, Xiaojue;Zhao, Daxian
开发安全有效的疫苗以预防高致病性H5N1禽流感病毒感染是一项具有挑战性的目标。乳酸乳球菌（Lactococcus lactis， L. lactis）是一种理想的疫苗递送载体，先前的研究表明，口服免疫包封的乳酸乳球菌-血凝素（Lactococcus lactis， HA）可以在小鼠模型中提供完全的保护，抵御同源H5N1病毒的攻击。虽然鼻内免疫是一种很有吸引力的方法，但现在有报道称，分泌性乳杆菌- ha与粘膜佐剂热不稳定毒素B亚单位（LTB）联合可以在鸡模型中提供保护性免疫。与乳杆菌ha单独鼻内免疫相比，乳杆菌ha联合LTB（乳杆菌ha +LTB）在接种鸡体内可引起较强的中和抗体反应和黏膜IgA反应，以及较强的细胞免疫反应。重要的是，乳杆菌- ha +LTB鼻内免疫可以提供100%的保护，以抵御H5N1病毒的攻击。综上所述，这些结果表明，在禽流感A/H5N1大流行期间，乳酸菌- ha +LTB鼻内免疫可被认为是预防和控制家禽感染H5N1病毒的有效方法。中华医学杂志，2015,31(4):391 - 391。(c) 2014 Wiley期刊公司
Development of safe and effective vaccines to prevent highly pathogenic avian influenza H5N1 virus infection is a challenging goal. Lactococcus lactis (L. lactis) is an ideal delivery vector for vaccine development, and it has been shown previously that oral immunization of encapsulated secretory L. lactis-hemagglutinin (HA) could provide complete protection against homologous H5N1 virus challenge in the mice model. While intranasal immunization is an appealing approach, it is now reported that secretory L. lactis-HA combined with mucosal adjuvant heat-labile toxin B subunit (LTB) could provide protective immunity in the chicken model. As compared to intranasal immunization with L. lactis-HA alone, L. lactis-HA combined with LTB (L. lactis-HA+LTB) could elicit robust neutralizing antibody responses and mucosal IgA responses, as well as strong cellular immune responses in the vaccinated chickens. Importantly, intranasal immunization with L. lactis-HA+LTB could provide 100% protection against H5N1 virus challenge. Taken together, these results suggest that intranasal immunization with L. lactis-HA+LTB can be considered as an effective approach for preventing and controlling infection of H5N1 virus in poultry during an avian influenza A/H5N1 pandemic. J. Med. Virol. 87: 39-44, 2015. (c) 2014 Wiley Periodicals, Inc.