Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps
Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps
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DOI：
10.1016/j.jaci.2008.03.002
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发表时间：
2008-06-01
影响因子：
14.2
通讯作者：
Schleimer, Robert P.
中科院分区：
文献类型：
--
作者：
Kato, Atsushi;Peters, Anju;Schleimer, Robert P.
背景：息肉型慢性鼻-鼻窦炎(慢性鼻-鼻窦炎合并鼻息肉[CRSwNP])是一种非常常见的疾病，通常需要手术治疗。鼻息肉中含有大量的B淋巴细胞、免疫球蛋白和嗜酸性粒细胞。目的：研究慢性鼻窦炎(CRS)患者鼻腔组织和鼻腔灌洗液中肿瘤坏死因子家族B细胞激活因子(BAFF)的表达。结果：慢性鼻咽癌患者鼻息肉组织中BAFF、B淋巴细胞标志物、CD20及跨膜激活物、钙调节剂和亲环素配体相互作用蛋白的表达采用实时定量聚合酶链式反应检测，BAFF蛋白的表达采用免疫组织化学方法检测。
Background: The polypoid form of chronic rhinosinusitis (chronic rhinosinusitis with nasal polyps [CRSwNP]) is a highly prevalent disease that often requires surgical intervention for treatment. Nasal polyps contain large quantities of B lymphocytes and immunoglobulin as well as eosinophils.Objectives: The objective of this study was to investigate the expression of B cell-activating factor of the TNF family (BAFF), an important regulator of class-switch recombination and immunoglobulin production, in patients with chronic rhinosinusitis (CRS).Methods: We collected nasal tissue and nasal lavage fluid from patients with CRS and control subjects. We assayed mRNA for BAFF and B-lymphocyte markers, CD20 and transmembrane activator and calcium-modulator and cyclophilin ligand interactor, by using real-time PCR, and assayed BAFF protein by using ELISA and immunohistochemistry.Results: BAFF mRNA was significantly increased in nasal polyps from patients with CRSwNP (P