An Optochemical Oxygen Scavenger Enabling Spatiotemporal Control of Hypoxia
An Optochemical Oxygen Scavenger Enabling Spatiotemporal Control of Hypoxia
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一种能够时空控制缺氧的光化学除氧剂
DOI：
10.1002/anie.202217585
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Nakagawa Hidehiko
中科院分区：
文献类型：
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作者：
Ieda Naoya;Sawada Masato;Oguchi Runa;Itoh Masato;Hirakata Seina;Saitoh Daisuke;Nakao Akito;Kawaguchi Mitsuyasu;Sawamoto Kazunobu;Yoshihara Toshitada;Mori Yasuo;Nakagawa Hidehiko
我们提出了一个光化学O2清除系统，使精确的时空控制的缺氧水平在活细胞中简单地通过调节光照区域的光强度。该系统采用含有硒或碲原子的罗丹明作为光化学氧清除剂，其在可见光照射（560-590 nm）时通过与作为共还原剂的谷胱甘肽的光化学反应快速消耗O2，并且具有快速的响应时间，在几分钟内。 系统的谷胱甘肽消耗量子产率计算为约5%。利用低氧响应荧光探针MAR观察培养细胞的氧消耗时空变化，并应用磷光寿命成像技术证实不同光强可产生不同程度的低氧。为了说明该系统在缺氧研究中的潜在效用，我们表明它可以时空控制钙离子（Ca 2+）流入表达缺氧响应性Ca 2+通道TRPA 1的HEK 293 T细胞。
We present an optochemical O2scavenging system that enables precise spatiotemporal control of the level of hypoxia in living cells simply by adjusting the light intensity in the illuminated region. The system employs rhodamine containing a selenium or tellurium atom as an optochemical oxygen scavenger that rapidly consumes O2by photochemical reaction with glutathione as a coreductant upon visible light irradiation (560–590 nm) and has a rapid response time, within a few minutes. The glutathione‐consuming quantum yields of the system were calculated as about 5 %. The spatiotemporal O2consuming in cultured cells was visualized with a hypoxia‐responsive fluorescence probe, MAR. Phosphorescence lifetime imaging was applied to confirmed that different light intensities could generate different levels of hypoxia. To illustrate the potential utility of this system for hypoxia research, we show that it can spatiotemporally control calcium ion (Ca2+) influx into HEK293T cells expressing the hypoxia‐responsive Ca2+channel TRPA1.