Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2)
Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2)
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DOI：
10.1111/j.1438-8677.2010.00409.x
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发表时间：
2011-03-01
期刊：
影响因子：
3.9
通讯作者：
Yazaki, K.
中科院分区：
文献类型：
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作者：
Koeduka, T.;Shitan, N.;Yazaki, K.
香精是在许多植物中发现的天然化合物，包括重要的水果作物番茄。异戊烯化类黄酮由一大组化合物组成，其通常表现出抗肿瘤、抗菌和/或抗雄激素活性。在这项研究中，我们设计的生物合成异戊烯化类黄酮使用链霉菌异戊烯基转移酶HypSc（SCO 7190）具有广泛的底物特异性，在番茄作为宿主植物。LC/MS/MS分析表明，当重组HypSc蛋白靶向质体时，番茄果实中产生3 '-二甲基烯丙基柚皮素，而重组蛋白在体外几乎不产生该化合物。这是第一份报告证实的异戊二烯化类黄酮的积累，使用细菌异戊二烯基转移酶在转基因植物中，我们的研究结果表明，异戊二烯基转移酶的产品特异性可以显着影响宿主植物。
Flavonoids are natural compounds found in many plants, including the important fruit crop, tomato. Prenylated flavonoids consist of a large group of compounds, which often exhibit antitumour, antibacterial and/or anti-androgen activities. In this study, we engineered the biosynthesis of prenylated flavonoids using a Streptomyces prenyltransferase HypSc (SCO7190) possessing broad-range substrate specificity, in tomato as a host plant. LC/MS/MS analysis demonstrated the generation of 3'-dimethylallyl naringenin in tomato fruits when recombinant HypSc protein was targeted to the plastids, whereas the recombinant protein hardly produced this compound in vitro. This is the first report confirming the accumulation of a prenylated flavonoid using a bacterial prenyltransferase in transgenic plants, and our results suggest that the product specificities of prenyltransferases can be significantly influenced by the host plant.