Profiling of phytohormones in rice under elevated cadmium concentration levels by magnetic solid-phase extraction coupled with liquid chromatography tandem mass spectrometry
Profiling of phytohormones in rice under elevated cadmium concentration levels by magnetic solid-phase extraction coupled with liquid chromatography tandem mass spectrometry
复制标题
通过磁固相萃取结合液相色谱串联质谱法分析高镉浓度水平下稻米中的植物激素
DOI：
10.1016/j.chroma.2015.06.046
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发表时间：
2015-08-07
影响因子：
4.1
通讯作者：
Feng, Yu-Qi
中科院分区：
文献类型：
--
作者：
Cai, Bao-Dong;Yin, Jia;Feng, Yu-Qi
植物激素是具有多种结构的信号小分子的集合，调控着植物的一系列生理过程。例如，它们调节生长发育，对生物和非生物胁迫的反应。痕量内源植物激素的定量研究是阐明其响应胁迫的分子机制所必需的。然而，由于植物激素结构和化学成分的多样性，对多种植物激素的提纯和一步富集存在困难。本研究建立了一种利用Fe3O4@TiO2-based磁固相萃取-超高效液相色谱-电喷雾串联质谱（Fe3O4@TiO2-based MSPE-UPLC-MS/MS）对8种细胞分裂素（CKs）、吲哚-3-乙酸（IAA）、脱落酸（ABA）、茉莉酸（JA）和10种赤霉素（GAs）等植物激素进行综合分析的方法。在该方法中，在UPLC-MS/MS分析之前，使用Fe3O4@TiO2作为吸附剂，通过一步MSPE捕获和纯化植物组织乙腈提取物中的植物激素。结果表明，该分析方法的灵敏度、准确性和重现性均能满足植物组织中多种激素的分析要求。利用Fe3O4@TiO2-based MSPE-UPLC-MS/MS方法研究Cd胁迫下水稻体内植物激素的变化。结果表明，在Cd胁迫下，植物的ck、IAA、ABA、JA和生物活性GAs均增加，表明这些植物激素可能参与了对Cd胁迫的响应。该研究有助于进一步了解植物激素在Cd胁迫下的生理功能。(C) 2015 Elsevier B.V.版权所有
Phytohormones, a collection of signal small molecules with various structures, regulate a series of physiological processes of plants. For instance, they regulate the growth and development, response to biotic and abiotic stresses. Quantification of trace endogenous phytohormones is essential to elucidate their molecular mechanisms in response to stresses. However, the structural and chemical diversity of phytohormones make it difficult to purify and enrich multiple phytohormones inane-step. In the current study, a method was developed to comprehensively profile phytohormones, including 8 cytokinins (CKs), indole-3-acetic acid (IAA), abscisic acid (ABA), jasmonic acid (JA) and 10 gibberellins (GAs) by Fe3O4@TiO2-based magnetic solid-phase extraction coupled with ultra-performance liquid chromatography-electrospray tandem mass spectrometry (Fe3O4@TiO2-based MSPE-UPLC-MS/MS). In the proposed method, the phytohormones in the acetonitrile extract of plant tissues were captured and purified by one-step MSPE using Fe3O4@TiO2 as a sorbent prior to UPLC-MS/MS analysis. The sensitivity, accuracy and reproducibility of the proposed analytical method were demonstrated to satisfy the profiling of multiple phytohormones in plant tissue. We then further used the Fe3O4@TiO2-based MSPE-UPLC-MS/MS method to explore the change of phytohormones in rice under Cd stress. The results showed that CKs, IAA, ABA, JA and biological active GAs all increased under Cd stress, suggesting that these phytohormones may take part in response to Cd stress. The study may promote the further understanding of the physiological functions of phytohormones in response to Cd stress. (C) 2015 Elsevier B.V. All rights reserved.