Probabilistic models for collecting genetic diversity: Comparisons, caveats, and limitations
Probabilistic models for collecting genetic diversity: Comparisons, caveats, and limitations
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DOI：
10.2135/cropsci2006.04.0262
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发表时间：
2007-03-01
期刊：
影响因子：
2.3
通讯作者：
Volk, Gayle M.
中科院分区：
文献类型：
--
作者：
Lockwood, Dale R.;Richards, Christopher M.;Volk, Gayle M.
从原位种群中收集遗传多样性的方法是植物保护的重要工具。已提出了许多定量采集策略，用于在没有关于生态、生殖生物学或种群遗传结构的先验信息的情况下采样种群，但它们对采集规模和多样性基础的不同假设往往使它们难以比较。了解不同策略的局限性，使收集者能够在实施保护和修复项目或收集种质改良时做出更明智的选择。我们在一组共同的假设下比较了两种基于遗传的策略，并扩展了策略的概率论证，以适应罕见的等位基因、多个基因座和多个群体。许多模型的建议是基于单个基因座的，但必须收集更多的个体，以确保在一个群体内的多个独立基因座上获得等位基因的概率很高(0.95)。对通过基因流动联系在一起的多个种群进行采样可能会抵消这一增长。此外，当对多个基因座或跨多个群体进行采样时，捕获稀有等位基因的可能性仍然很高。这些模型的比较为种质收集者提供了一个基础，以评估过度和不足采样的风险，以获取物种内的遗传多样性。
Methods for collecting genetic diversity from in situ populations are important tools for plant conservation. Many quantitative collection strategies for sampling populations without a priori information regarding the ecology, reproductive biology, or population genetic structure of the taxa have been proposed, but their different assumptions regarding the collection scale and the basis for diversity often make them difficult to compare. Understanding the limitations of the different strategies enables collectors to make more informed choices when implementing conservation and restoration projects or collecting for germplasm improvement. We compare two genetically based strategies under a common set of assumptions and extend the probabilistic arguments of the strategies to accommodate rare alleles, multiple loci, and multiple populations. The recommendations of many models are based on a single locus, but larger numbers of individuals must be collected to assure with a high probability (> 0.95) the acquisition of alleles at multiple independent loci within a population. Sampling from multiple populations linked by gene flow may offset this increase. Additionally, the likelihood of capturing rare alleles remains high when sampling for multiple loci or across multiple populations. Comparison of the models provides germplasm collectors with a basis to evaluate risks of over- and undersampling to capture genetic diversity within a species.