Ultraviolet-B recruits mannose-binding lectin into skin from non-cutaneous sources.
Ultraviolet-B recruits mannose-binding lectin into skin from non-cutaneous sources.
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DOI：
10.1111/j.0022-202x.2005.23794.x
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发表时间：
2005-07
期刊：
影响因子：
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通讯作者：
Michele L Lokitz;Wei Zhang;M. Bashir;K. Sullivan;G. Ang;E. J. Kwon;Julie H. Lin;V. Werth
中科院分区：
文献类型：
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作者：
Michele L Lokitz;Wei Zhang;M. Bashir;K. Sullivan;G. Ang;E. J. Kwon;Julie H. Lin;V. Werth
甘露糖结合凝集素（MBL）是先天免疫系统的组成部分，并且作为调理素通过结合病原体和某些凋亡细胞以促进它们被吞噬细胞摄取而起作用。我们最近确定了一个协会的低生产MBL多态性与成人皮肌炎（DM）。我们的模型是MBL缺乏导致皮肤中细胞凋亡碎片的清除缺陷，从而诱发光敏性自身免疫性疾病。在这项研究中，我们试图确定MBL是否在表皮内结合，并确定其来源和这种结合的潜在功能。我们证明，MBL是存在于照射，但不是在非照射皮肤，在照射皮肤，它是结合到凋亡角质形成细胞（KC）。我们发现MBL不是由KC制造的，间接地表明它来自外源性来源，尽管其他补体成分是由KC制造的并且被紫外线照射上调。最后，我们证明了非KC衍生的MBL在体外与凋亡KC结合，并增加了树突状细胞对这些细胞的摄取。我们推测MBL可能有助于光敏型DM患者凋亡碎片的非炎症清除。
Mannose-binding lectin (MBL) is an integral part of the innate immune system and functions as an opsonin by binding to pathogens and certain apoptotic cells to promote their uptake by phagocytes. We recently identified an association of low-producing MBL polymorphisms with adult dermatomyositis (DM). Our model is that MBL deficiency leads to a defect in the clearance of apoptotic debris in the skin, thereby predisposing to photosensitive autoimmune disease. In this study, we sought to determine whether MBL binds within the epidermis, and to determine its source, and potential function of this binding. We demonstrated that the MBL is present in irradiated, but not in non-irradiated skin, and in irradiated skin it is bound to apoptotic keratinocytes (KC). We found that MBL is not made by KC, showing indirectly that it comes from an exogenous source, despite the fact that other complement components are made by KC and upregulated by ultraviolet irradiation. Finally, we demonstrated that non-KC-derived MBL bound to apoptotic KC in vitro and increased the uptake of these cells by dendritic cells. We hypothesize that MBL may facilitate non-inflammatory clearance of apoptotic debris in patients with photosensitive forms of DM.