Herpesvirus papio 2 (HVP2): sequence analysis of the unique short (US) region.
Herpesvirus papio 2 (HVP2): sequence analysis of the unique short (US) region.
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狒狒疱疹病毒 2 (HVP2)：独特短 (US) 区域的序列分析。
DOI：
10.1007/s11262-005-6878-0
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发表时间：
2006
期刊：
影响因子：
1.6
通讯作者：
Martin,DavidW
中科院分区：
文献类型：
--
作者：
Bigger,JohnE;Martin,DavidW
狒狒感染狒狒病毒 2 (HVP2) 会产生一种临床上与人类感染单纯疱疹病毒 (HSV) 相似的疾病（[1]，D. Martin，未发表）。此外，之前的研究表明，HVP2基因组大小相似，并与单纯疱疹病毒1型和2型（HSV1和HSV2）、猿猴病毒8（SA8）和B型疱疹病毒（B病毒）共线。 HVP2 基因组包含由反向重复 DNA 区域分隔的独特长 (UL) 和独特短 (US) 区域。迄今为止，已经分析了大约 27 个部分开放阅读框和 4 个完整开放阅读框，所有这些都证明了 HVP2 蛋白与其 HSV 同源物之间的高度同一性 [2, 3]。 HVP2 和 HSV 在临床和遗传水平上的相似性表明，HVP2 系统有潜力成为一个强大的替代模型，用于研究自然灵长类宿主中单纯疱疹病毒复制的生物学。为了继续我们对 HVP2 的表征并确定进一步研究的靶标，我们确定了 HVP2 基因组的独特短 (US) 区域的 DNA 序列。用 HVP2 分离株 860 感染 Vero 细胞，并通过氯化铯梯度离心纯化 DNA。使用 ABI 技术对覆盖美国大部分地区的基因组克隆的两条链进行了测序。 US6 KpnI 位点（核苷酸 7822）和 PstI 位点（核苷酸 8627）之间的一个小区域（405 bp）是
Infection of baboons with herpesvirus papio 2 (HVP2) produces a disease that is clinically similar to herpes simplex virus (HSV) infection of humans ([1], D. Martin, unpublished). In addition, previous studies have demonstrated that the HVP2 genome is similar in size and collinear with the herpes simplex virus types 1 and 2 (HSV1 and HSV2), simian agent 8 (SA8), and herpes B virus (B virus). The HVP2 genome contains unique long (UL) and unique short (US) regions separated by regions of inverted repeat DNA. To date some 27 partial and four complete open reading frames have been analyzed, all of which demonstrate high degrees of identity between the proteins of HVP2 and their HSV homologues [2, 3]. The similarity between HVP2 and HSV at both the clinical and genetic levels indicates that the HVP2 system has the potential to be a powerful surrogate model to study the biology of herpes simplex virus replication in the context of a natural primate host.In order to continue our characterization of HVP2 and to identify targets for further study, we have determined the DNA sequence of the unique short (US) region of the HVP2 genome. Vero cells were infected with HVP2 isolate 860 and the DNA was purified by cesium chloride gradient centrifugation. Genomic clones covering the majority of US were sequenced on both strands using ABI technology. A small (405 bp) region of US6 between the KpnI site (nucleotide 7822) and the PstI site (nucleotide 8627) was