CREATININE ASSAY BY A REACTION-KINETIC PRINCIPLE
CREATININE ASSAY BY A REACTION-KINETIC PRINCIPLE
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DOI：
10.1016/0009-8981(72)90513-x
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发表时间：
1972-01-01
影响因子：
5
通讯作者：
LARSEN, K
中科院分区：
文献类型：
--
作者：
LARSEN, K
本文描述了一种不需要脱蛋白的具有Jaffé反应的血清和尿肌酐的反应动力学分析方法。考察了所有反应参数，找出了应用反应动力学原理的最佳条件。反应过程在第一分钟表现出比随后几分钟更大的pH依赖性，血清蛋白的缓冲能力降低了反应速度，与无血清反应混合物中的反应速度相比，可以通过使用合适的血清恒定因子进行修正。在缓冲作用之外，蛋白质不会影响反应速度，因此不需要脱蛋白。血清可能含有Jaffé阳性化合物--反应速度快于肌酐--例如乙酰乙酸酯--也可能含有反应速度慢于肌酐的化合物。在第二分钟内的测量结果与使用劳埃德试剂的非动力学方法相当。第一分钟的测定值比第二分钟的测定值平均高出约17μ摩尔/升，但比不含劳埃德试剂的非动力学方法的测定值低14μ摩尔/L。用LKB8600反应速率分析仪进行的反应动力学原理非常简单，并且被证明是非常可靠的。每个样品所需的时间为12-2分钟，这取决于对精密度的要求，特别是对特异性的要求。第1分钟测量得到的参考(正常)范围：男性80-138mole/L，女性63-121mole/L；第2分钟：男性69-117mole/L，女性53-101μ/μ/L。
A reaction-kinetic assay of serum and urine creatinine with the Jaffé reaction but without deproteinization is described. All reaction parameters have been investigated to find optimal conditions for the application of the reaction-kinetic principle. The reaction course shows greater pH dependence during the first minute than during the following minutes and the buffer capacity of serum protein reduces the reaction rate relative to that in a serum-free reaction mixture; correction for this can be made by using a suitable constant factor for serum. Beyond the buffer effect protein does not influence the reaction rate; thus deproteinization is unnecessary. Serum may contain Jaffé-positive compounds reacting faster than creatinine—acetoacetate is an example—and also contain compounds reacting slower than creatinine. Measurements during the second minute give values comparable to non-kinetic methods with Lloyd's reagent. Values from measurements during the first minute are on an average about 17 μmole/1 higher than second minute values, but 14 μmole/l lower than by a non-kinetic method without Lloyd's reagent. The reaction-kinetic principle carried out with the LKB 8600 Reaction Rate Analyzer is very simple and has proved extremely reliable. The time required per sample is 1 2–2 min depending on demands for precision and especially for specificity. Reference (normal) ranges found by measurements during the first minute: males 80–138 μmole/l, females 63–121 μmole/l and during the second minute: males 69–117 μmole/l, females 53–101 μmole/l.