Using MESIMS to analyze polymer MALDI matrix solubility
Using MESIMS to analyze polymer MALDI matrix solubility
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DOI：
10.1016/j.jasms.2005.03.013
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发表时间：
2005-07-01
影响因子：
3.2
通讯作者：
Owens, KG
中科院分区：
文献类型：
--
作者：
Hanton, SD;Owens, KG
可靠的样品制备方法的开发对于基质辅助激光解吸/电离 (MALDI) 质谱实验的成功至关重要。良好的聚合物 MALDI 样品制备包括正确选择溶剂系统、基质和电离剂，并以能够产生所需离子的样品的方式将它们组合起来。工业聚合物中可用的化学成分多种多样，这对我们设计可靠的样品制备方法的能力提出了挑战。在此报告的实验中，我们表明基质增强二次离子质谱 (MESIMS) 是探索固相 MALDI 样品中样品分离的有效分析技术。对多种基质制备的聚合物样品的 MESIMS 和 MALDI 结果进行定性比较有助于我们研究各种低分子量聚合物材料的固相溶解度。将聚合物样品和基质的固相溶解度与液相溶解度结合起来，可以构建相对溶解度图，该图显示了 MALDI 实验中聚合物和基质材料之间的最佳溶解度匹配。 (c) 2005 年美国质谱学会。
The development of reliable sample preparation methods has been critical to the success of matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry experiments. Good MALDI sample preparation for polymers involves choosing the solvent system, the matrix, and the ionization agent correctly, and combining them in a manner that will lead to a sample that will produce the desired ions. The vast diversity of chemistry available in industrial polymers has challenged our ability to design reliable sample preparation methods. In the experiments reported here, we show that matrix-enhanced secondary ion mass spectrometry (MESIMS) is an effective analytical technique to explore sample segregation in solid phase MALDI samples. Qualitative comparison of MESIMS and MALDI results for polymer samples prepared with multiple matrices aids our investigation of the solid-phase solubility of a variety of low molecular weight polymer materials. Including the solid-phase solubility with the liquid-phase solubility of the polymer samples and the matrices enables the construction of a relative solubility chart, which shows the best solubility matches between the polymer and matrix materials for MALDI experiments. (c) 2005 American Society for Mass Spectrometry.