Minimally immunogenic decellularized porcine valve provides in situ recellularization as a stentless bioprosthetic valve
Minimally immunogenic decellularized porcine valve provides in situ recellularization as a stentless bioprosthetic valve
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DOI：
10.1007/s10047-006-0360-1
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发表时间：
2007-01-01
影响因子：
1.3
通讯作者：
Sawa, Yoshiki
中科院分区：
文献类型：
--
作者：
Iwai, Shigemitsu;Torikai, Kei;Sawa, Yoshiki
目前使用的生物瓣膜有几个局限性，如钙化和功能恶化，通过细胞内长入进行振兴是不可能的。为了克服这些障碍，我们开发了一种免疫原性最低的组织工程瓣膜，它由一种无固定的、脱细胞的猪瓣支架组成，能够在植入后在体内自发恢复活力。用十二烷基硫酸钠和Triton X-100等洗涤剂对猪主动脉根部组织进行脱细胞处理。猪瓣膜得到了非常温和的处理，并允许成分在基质中扩散进入和流出。在初步研究中，将一块脱细胞猪瓣组织植入大鼠真皮下间隙14天和60天，并对结构完整性和钙化进行评估。作为活体瓣膜置换模型，将脱细胞猪瓣植入犬的肺动脉瓣部位，分别于术后1、2、6个月进行功能和组织学评价。组织学检查表明，新开发的洗涤剂处理方法有效地去除了猪主动脉组织中的细胞碎片。去细胞猪瓣组织植入大鼠皮下后，炎症细胞浸润和钙化程度最低。在瓣膜置换术模型中，2个月内中膜细胞自发再生和再生，超声心动图观察到6个月内瓣膜功能良好，无返流。最低免疫原性去细胞猪瓣膜被证明有效地减轻了术后钙化，并通过原位再细胞化、细胞内长入和组织重塑为修复假体提供了合适的基质。
Currently used bioprosthetic valves have several limitations such as calcification and functional deterioration, and revitalization through cellular ingrowth is impossible. To overcome these obstacles, we have developed a minimally immunogenic tissue-engineered valve that consists of an unfixed, decellularized porcine valve scaffold capable of being spontaneously revitalized in vivo after implantation. Porcine aortic root tissue was decellularized using detergents such as sodium lauryl sulfate and Triton X-100. The porcine valve was treated very gently and plenty of time was allowed for constituents to diffuse in and out of the matrix. In a preliminary study, a piece of decellularized porcine valve tissue was implanted into the rat subdermal space for 14 and 60 days and the structural integrity and calcification were evaluated. As an in vivo valve replacement model, the decellularized porcine valve was implanted in the pulmonary valve position in dogs and functional and histological evaluation was performed after 1, 2, and 6 months. Histological examination showed that the newly developed detergent treatment effectively removed cellular debris from the porcine aortic tissue. Decellularized porcine valve tissue implanted subdermally in rats showed minimal inflammatory cell infiltration and calcification. In the valve replacement model, spontaneous reen-dothelialization and repopulation of the medial cells were observed within 2 months, and good valve function without regurgitation was observed by echocardiography up to 6 months. The minimally immunogenic decellularized porcine valve proved effective in mitigating postimplant calcification and provided a suitable matrix for revitalizing prostheses through in situ recellularization, cellular ingrowth, and tissue remodeling.