Campylobacter pyloridis degrades mucin and undermines gastric mucosal integrity.
Campylobacter pyloridis degrades mucin and undermines gastric mucosal integrity.
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幽门弯曲杆菌降解粘蛋白并破坏胃粘膜完整性。
DOI：
10.1016/s0006-291x(87)80511-9
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发表时间：
1987
影响因子：
3.1
通讯作者：
Slomiany,A
中科院分区：
文献类型：
--
作者：
Slomiany,BL;Bilski,J;Sarosiek,J;Murty,VL;Dworkin,B;VanHorn,K;Zielenski,J;Slomiany,A
研究了幽门弯曲菌（一种与胃炎和消化性溃疡相关的螺旋菌）在削弱胃粘膜屏障粘液成分中的作用。从接受胃镜检查的患者的胃窦粘膜活检培养的细菌菌落用盐水洗涤，通过无菌过滤器，并检查滤液的蛋白酶和糖基水解酶活性。结果表明，该滤液不仅对白蛋白等典型蛋白质底物，而且对胃粘蛋白也表现出很强的蛋白水解活性。该酶在pH 7.0时对粘蛋白的降解最佳，在低分子量下具有蛋白酶活性。（<50K）蛋白质级分。滤液显示很少的糖基水解酶活性，并且不引起粘蛋白碳水化合物的水解。这些数据表明，幽门螺杆菌感染通过引起保护性粘液层的粘蛋白组分的蛋白水解降解来削弱胃粘膜防御。
The role of Campylobacter pyloridis, a spiral bacteria associated with gastritis and peptic ulcers in weakening the mucus component of gastric mucosal barrier was investigated. The colonies of bacteria, cultured from antral mucosal biopsies of patients undergoing gastroscopy, were washed with saline, passed through sterilization filter and the filtrate was examined for protease and glycosylhydrolase activities. The obtained results revealed that the filtrate exhibited a strong proteolytic activity not only towards the typical protein substrates such as albumin but also towards gastric mucin. Optimum enzymatic activity for degradation of mucin was attained at pH 7.0 and the protease activity was found in a low m.w. (<50K) protein fraction. The filtrate showed little glycosylhydrolase activity and did not cause the hydrolysis of mucin carbohydrates. The data suggest that C pyloridis infection weakens the gastric mucosal defense by causing proteolytic degradation of mucin component of the protective mucus layer.