The inositol polyphosphate 5-phosphatase Ocrl associates with endosomes that are partially coated with clathrin.
The inositol polyphosphate 5-phosphatase Ocrl associates with endosomes that are partially coated with clathrin.
复制标题
肌醇多磷酸 5-磷酸酶 Ocrl 与部分包被网格蛋白的内体结合。
DOI：
10.1073/pnas.0405664101
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发表时间：
2004
影响因子：
11.1
通讯作者：
Majerus,PhilipW
中科院分区：
文献类型：
--
作者：
Ungewickell,Alexander;Ward,MichaelE;Ungewickell,Ernst;Majerus,PhilipW
荧光显微镜研究了在Lowe综合征中突变的肌醇多磷酸5-磷酸酶ocl的亚细胞定位。在固定细胞中，ocl与网格蛋白、巨球蛋白、甘露糖6-磷酸受体、转铁蛋白和早期内体抗原1内体标记物一起定位于核内体和高尔基膜。通过活细胞延时显微镜，我们监测了ocl在核内体上的动态，证实了ocl的内体定位。GST结合实验表明，ocl与网格蛋白末端结构域和网格蛋白接头蛋白AP-2相互作用。我们的研究结果表明，ocl在内体受体运输和分类中的作用。
The subcellular localization of Ocrl, the inositol polyphosphate 5-phosphatase that is mutated in Lowe syndrome, was investigated by fluorescence microscopy. Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannose 6-phosphate receptor, transferrin, and the early endosomal antigen 1 endosomal marker in fixed cells. The endosomal localization of Ocrl was confirmed by live-cell time-lapse microscopy in which we monitored the dynamics of Ocrl on endosomes. GST binding assays show that Ocrl interacts with the clathrin terminal domain and the clathrin adaptor protein AP-2. Our findings suggest a role for Ocrl in endosomal receptor trafficking and sorting.