Extracellular ATP plays an important role in systemic wound response activation
Extracellular ATP plays an important role in systemic wound response activation
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DOI：
10.1093/plphys/kiac148
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发表时间：
2022-03-28
期刊：
影响因子：
7.4
通讯作者：
Mittler, Ron
中科院分区：
文献类型：
--
作者：
Myers, Ronald J.;Fichman, Yosef;Mittler, Ron
机械损伤发生在生物或非生物胁迫下的植物中，与触发全身组织损伤反应的远距离信号通路的激活有关。损伤激活的系统信号包括电信号、钙、水力和活性氧（ROS）波。最近有人提出，受损组织细胞释放谷氨酸（Glu）可通过谷氨酸样受体（GLRs）触发系统性信号转导通路。然而，细胞在受伤过程中释放的另一种重要化合物（细胞外ATP [eATP]）在触发全身反应中的作用尚不清楚。在这里，我们在拟南芥（Arabidopsis thaliana）中表明，损伤导致纳米摩尔水平的eATP积累，这些水平足以触发系统性ROS波。我们进一步表明，在受伤过程中，进食atp触发ROS波需要嘌呤受体2激酶（P2K）受体。在未受伤的叶片上施用eATP会触发ROS波，而在P2Ks缺陷突变体（如p2k1-3、p2k2和p2k1-3p2k2）中，施用eATP或损伤会抑制ROS波的激活。此外，在p2k缺失的突变体中，全身创伤反应（SWR）转录物的表达在损伤过程中受到抑制。有趣的是，Glu和eATP对ROS波激活的影响并不是相加的，这表明这两种化合物在同一途径上触发ROS波。我们的研究结果表明，除了通过glr感知Glu外，p2k感知的eATP在植物swr的触发中起着关键作用。细胞在受伤过程中释放的细胞外ATP触发系统活性氧信号和转录组反应。
Mechanical wounding occurs in plants during biotic or abiotic stresses and is associated with the activation of long-distance signaling pathways that trigger wound responses in systemic tissues. Among the different systemic signals activated by wounding are electric signals, calcium, hydraulic, and reactive oxygen species (ROS) waves. The release of glutamate (Glu) from cells at the wounded tissues was recently proposed to trigger systemic signal transduction pathways via GLU-LIKE RECEPTORs (GLRs). However, the role of another important compound released from cells during wounding (extracellular ATP [eATP]) in triggering systemic responses is not clear. Here, we show in Arabidopsis (Arabidopsis thaliana) that wounding results in the accumulation of nanomolar levels of eATP and that these levels are sufficient to trigger the systemic ROS wave. We further show that the triggering of the ROS wave by eATP during wounding requires the PURINORECEPTOR 2 KINASE (P2K) receptor. Application of eATP to unwounded leaves triggered the ROS wave, and the activation of the ROS wave by wounding or eATP application was suppressed in mutants deficient in P2Ks (e.g. p2k1-3, p2k2, and p2k1-3p2k2). In addition, expression of systemic wound response (SWR) transcripts was suppressed in mutants deficient in P2Ks during wounding. Interestingly, the effect of Glu and eATP application on ROS wave activation was not additive, suggesting that these two compounds function in the same pathway to trigger the ROS wave. Our findings reveal that in addition to sensing Glu via GLRs, eATP sensed by P2Ks plays a key role in the triggering of SWRs in plants.Extracellular ATP released from cells during wounding triggers systemic reactive oxygen species signaling and transcriptomic responses.