A simple method for DNA extraction from marine bacteria that produce extracellular materials
A simple method for DNA extraction from marine bacteria that produce extracellular materials
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DOI：
10.1016/s0167-7012(02)00180-x
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发表时间：
2003-02-01
影响因子：
2.2
通讯作者：
Lee, HK
中科院分区：
文献类型：
--
作者：
Lee, YK;Kim, HW;Lee, HK
我们提出了一种从海洋细菌 Hahella chejuensis、Streptomyces sp. 和 Cytophaga sp. 中提取 DNA 的简单方法。此前，这些菌株的 DNA 纯化因细胞外物质的存在而受到阻碍。在我们的提取方法中，通过在液氮中冷冻和研磨来裂解海洋细菌，并用 SDS 进行处理。使用苯酚/氯仿混合物纯化提取的DNA，并在异丙醇中沉淀。提取的 DNA 质量高，适合分子分析，例如 PCR、限制性内切酶消化、基因组 DNA 印迹杂交和基因组 DNA 文库构建。我们使用这种方法从其他几种海洋细菌中提取基因组 DNA。我们的方法是一种可重复、简单且快速的技术，用于从海洋细菌中常规提取 DNA。此外，该方法的低成本使其对于大规模研究具有吸引力。 (C) 2003 Elsevier Science B.V. 保留所有权利。
We present a simple method for extracting DNA from the marine bacteria Hahella chejuensis, a Streptomyces sp., and a Cytophaga sp. Previously, DNA purification from these strains was hindered by the presence of extracellular materials. In our extraction method, the marine bacteria are lysed by freezing and grinding in liquid nitrogen, and treated with SDS. The extracted DNA is purified using a phenol/chloroform mixture, and precipitated in isopropanol. The extracted DNA is of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion, genomic DNA blot hybridization, and genomic DNA library construction. We used this method to extract genomic DNA from several other marine bacteria. Our method is a reproducible, simple, and rapid technique for routine DNA extractions from marine bacteria. Furthermore, the low cost of this method makes it attractive for large-scale studies. (C) 2003 Elsevier Science B.V. All rights reserved.