Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: A major site of neurogenesis
Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: A major site of neurogenesis
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DOI：
10.1073/pnas.0308600100
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发表时间：
2004-03-02
影响因子：
11.1
通讯作者：
Huttner, WB
中科院分区：
文献类型：
--
作者：
Haubensak, W;Attardo, A;Huttner, WB
哺乳动物中枢神经系统的神经元被认为起源于在神经上皮顶端表面分裂的祖细胞。在此，我们利用从Tis21基因座表达绿色荧光蛋白的小鼠胚胎来特异性地揭示产生中枢神经系统神经元的细胞分裂。Tis21基因在大多数（如果不是全部）产生神经元的祖细胞中在整个神经管中表达。除了神经上皮（NE）细胞在顶端的不对称分裂（产生另一个NE细胞和一个神经元）之外，我们从神经发生开始就发现了第二群祖细胞，它们在神经上皮的基底区域分裂并产生两个神经元。基底祖细胞在端脑中最为常见，在那里它们的数量超过了在顶端分裂产生神经元的NE细胞。我们的观察结果调和了先前关于中枢神经系统神经元起源和谱系的数据，并表明基底祖细胞而非顶端祖细胞是哺乳动物新皮质神经元的主要来源。
Neurons of the mammalian CNS are thought to originate from progenitors dividing at the apical surface of the neuroepithelium. Here we use mouse embryos expressing GFP from the Tis21 locus, a gene expressed throughout the neural tube in most, if not all, neuron-generating progenitors, to specifically reveal the cell divisions that produce CNS neurons. In addition to the apical, asymmetric divisions of neuroepithelial (NE) cells that generate another NE cell and a neuron, we find, from the onset of neurogenesis, a second population of progenitors that divide in the basal region of the neuroepithelium and generate two neurons. Basal progenitors are most frequent in the telencephalon, where they outnumber the apically dividing neuron-generating NE cells. Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex.