Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination
Wastewater used for urban agriculture in West Africa as a reservoir for antibacterial resistance dissemination
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DOI：
10.1016/j.envres.2018.09.022
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发表时间：
2019-01-01
影响因子：
8.3
通讯作者：
Piddock, Laura J. V.
中科院分区：
文献类型：
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作者：
Bougnom, Blaise P.;Zongo, Cheikna;Piddock, Laura J. V.
利用最先进的宏基因组学技术调查瓦加杜古（布基纳法索）城市农业废水中的微生物种群、抗生素耐药基因和具有医学意义的质粒。废水样本采集自三个社区农田附近的三条运河。微生物种群多样性评估显示，不同样品的微生物模式不同。从废水中测序结果显示微生物群落的不同功能特化，以碳水化合物和蛋白质代谢功能为主。在废水样品中检测到11个病原体特异性基因和56个同源毒力因子基因。这些毒力因子通常存在于引起肠胃炎和/或腹泻的人类病原体中。鉴定出广泛的抗生素耐药基因；81种可通过移动遗传因子传播。其中包括7个不同的扩展谱β -内酰胺酶基因，编码4个酶家族的合成，包括两个金属β -内酰胺酶（bla（AIM-1）和bla(GES-21)）。10个肠杆菌科质粒复制子不相容组（ColE、FIB、FIC、FIL P、Q、R、U、Y和A/C）和30个革兰氏阳性菌质粒复制子类型。所有这些都与抗生素耐药基因的广泛分布有关。我们得出的结论是，城市中用于城市农业的废水具有在人类和动物中传播细菌和抗微生物药物耐药性的高风险。
State of art metagenomics were used to investigate the microbial population, antibiotic resistance genes and plasmids of medical interest in wastewater used for urban agriculture in Ouagadougou (Burkina Faso). Wastewater samples were collected from three canals near agricultural fields in three neighbourhoods. Assessment of microbial population diversity revealed different microbial patterns among the different samples. Sequencing reads from the wastewaters revealed different functional specializations of microbial communities, with the predominance of carbohydrates and proteins metabolism functions. Eleven pathogen-specific and 56 orthologous virulence factor genes were detected in the wastewater samples. These virulence factors are usually found in human pathogens that cause gastroenteritis and/or diarrhoea. A wide range of antibiotic resistance genes was identified; 81 are transmissible by mobile genetic elements. These included seven different extended spectrum beta-lactamase genes encoding synthesis of four enzyme families, including two metallo-beta-lactamases (bla(AIM-1) and bla(GES-21)). Ten different incompatibility groups of Enterobacteriaceae plasmid replicons (ColE, FIB, FIC, FIL P, Q, R, U, Y, and A/C), and 30 plasmid replicon types from Gram-positive bacteria. All are implicated in the wide distribution of antibiotic resistance genes. We conclude that wastewater used for urban agriculture in the city represents a high risk for spreading bacteria and antimicrobial resistance among humans and animals.