Influence of carbon nanomaterial defects on the formation of protein corona.
Influence of carbon nanomaterial defects on the formation of protein corona.
复制标题
碳纳米材料缺陷对蛋白质冠形成的影响。
DOI：
10.1039/c5ra15007h
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Podila,Ramakrishna
中科院分区：
文献类型：
--
作者：
Sengupta,Bishwambhar;Gregory,WrenE;Zhu,Jingyi;Dasetty,Siva;Karakaya,Mehmet;Brown,JaredM;Rao,ApparaoM;Barrows,JohnK;Sarupria,Sapna;Podila,Ramakrishna
在任何生理介质中，碳纳米材料（CNM）与生物分子强烈相互作用导致生物冕的形成，生物冕随后决定了CNM的生理反应和命运。CNMs中的缺陷不仅对材料性能起着重要的作用，而且对材料在纳米生物界面上的相互作用也起着重要的作用。在本文中，我们利用微拉曼、光致发光、红外光谱、电化学和分子动力学模拟探讨了缺陷诱导的亲水性对生物日冕形成的影响。我们的研究结果表明，蛋白质（白蛋白和纤维蛋白原）与cnm的相互作用受到它们之间电荷转移的强烈影响，诱导蛋白质展开，从而增强构象熵和更高的蛋白质吸附。
In any physiological media, carbon nanomaterials (CNM) strongly interact with biomolecules leading to the formation of biocorona, which subsequently dictate the physiological response and the fate of CNMs. Defects in CNMs play an important role not only in material properties but also in the determination of how materials interact at the nano-bio interface. In this article, we probed the influence of defect-induced hydrophilicity on the biocorona formation using micro-Raman, photoluminescence, infrared spectroscopy, electrochemistry, and molecular dynamics simulations. Our results show that the interaction of proteins (albumin and fibrinogen) with CNMs is strongly influenced by charge-transfer between them, inducing protein unfolding which enhances conformational entropy and higher protein adsorption.