PROTEIN HYDRATION STUDIED WITH HOMONUCLEAR 3D PROTON NMR EXPERIMENTS
PROTEIN HYDRATION STUDIED WITH HOMONUCLEAR 3D PROTON NMR EXPERIMENTS
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DOI：
10.1007/bf01877232
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发表时间：
1991-01-01
影响因子：
2.7
通讯作者：
WUTHRICH K
中科院分区：
文献类型：
--
作者：
OTTING G;LIEPINSH E;WUTHRICH K
同核三维1H NOESY - TOCSY和三维1H ROESY - TOCSY实验被用于解析和指认碱性胰蛋白酶抑制剂在H₂O溶液中水分子信号与各个多肽质子共振之间的核奥弗豪泽效应（NOE）交叉峰。结合一种新颖、稳健的水抑制技术，在4℃下检测到了正的和负的分子间NOE。观察到水质子和蛋白质质子之间的正NOE，能够更精确地估计水分子在蛋白质表面水合位点的极短停留时间。
Homonuclear 3D 1H NOESY-TOCSY and 3D 1H ROESY-TOCSY experiments were used to resolve and assign nuclear Overhauser effect (NOE) cross peaks between the water signal and individual polypeptide proton resonances in H2O solutions of the basic pancreatic trypsin inhibitor. Combined with a novel, robust water-suppression technique, positive and negative intermolecular NOEs were detected at 4.degree. C. The observation of positive NOEs between water protons and protein protons enables more precise estimates of the very short residence times of the water molecules in the hydration sites on the protein surface.