Interactive effects of testosterone and superior cervical ganglionectomy on attraction thresholds to volatile urinary odors in gonadectomized mice.
Interactive effects of testosterone and superior cervical ganglionectomy on attraction thresholds to volatile urinary odors in gonadectomized mice.
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睾酮和颈上神经节切除术对去腺小鼠挥发性尿液气味吸引阈值的相互作用。
DOI：
10.1016/s0166-4328(03)00073-1
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发表时间：
2003
影响因子：
2.7
通讯作者：
Baum,MJ
中科院分区：
文献类型：
--
作者：
Pankevich,DE;Deedy,EM;Cherry,JA;Baum,MJ
挥发性尿味有助于小鼠在主嗅觉上皮(MOE)检测到它们后识别配偶。我们使用习惯化/去习惯化任务来询问去性腺小鼠检测和研究繁殖雄性或发情雌性的挥发性尿味浓度下降的能力是否受到雄激素或雌激素的调节，如果是的话，这些性类固醇的任何作用是否通过双侧颈上神经节切除术(SCGx)扰乱MOE的交感神经来改变。在给出的测试中，在没有类固醇治疗的情况下，性腺切除后51天开始，男性和女性受试者都检测到了即使是最低浓度的男性尿味(按体积稀释1：120和1：160)，前提是它们是SCGx而不是假手术。在随后进行的5次α-双氢睾酮治疗后进行的测试中，无论男性还是女性受试者都没有检测到低浓度的男性尿味，无论他们的SCG是否完好无损。在最后一系列测试之前给予睾酮(T)恢复了性腺切除受试者的能力，无论他们的SCG状态如何，都能检测出低浓度的男性尿味。这表明T，而不是它的神经代谢物雌二醇，或DHT，促进了两性小鼠对低浓度雄性气味的反应。在没有类固醇治疗的性腺切除后51天的测试中，大多数男性和女性受试者很容易检测到发情女性尿味的三个最高浓度，而SCGx男性和女性未能检测到这些气味的最低浓度。在用EB和DHT治疗后，性腺切除的小鼠，无论其SCG状态如何，通常都无法检测到发情期女性尿味的三个最低浓度。在T处理后；然而，如果他们的SCG完好无损，男女受试者再次检测到发情女性尿液的大部分稀释。同样，这些结果表明，T，而不是雌二醇或DHT，促进了对发情女性尿味的反应。如果存在T的这种激活作用，交感神经、去甲肾上腺素对MOE的输入可能会增强气味对比度，如先前所建议的[NAT。神经科。2(1999)106]，通过将嗅觉神经元的反应性降低到低浓度(1：120和1：160稀释)一些具有生物学意义的气味(例如男性尿味)，同时促进它们对低到中等浓度(1：80稀释)的其他气味(例如发情期女性尿味)的反应性。
Volatile urinary odors contribute to mate recognition in mice after their detection by the main olfactory epithelium (MOE). We used a habituation/dishabitution task to ask whether the capacity of gonadectomized mice of both sexes to detect and investigate decreasing concentrations of volatile urinary odors from either breeding males or estrous females is modulated by administering androgen or estrogen and if so, whether any effects of these sex steroids are altered by disrupting the sympathetic innervation of the MOE via bilateral superior cervical ganglionectomy (SCGx). In tests given, beginning 51 days after gonadectomy without steroid treatment both male and female subjects detected even the lowest concentrations (1:120 and 1:160 dilutions by volume) of male urinary odors, provided they were SCGx as opposed to sham operated. In subsequent tests given after estradiol benzoate (EB) followed later by 5α-dihydrotestosterone (DHT) treatments, neither male nor female subjects detected low concentrations of male urinary odors regardless of whether or not their SCG’s were intact. Administration of testosterone (T) prior to a final series of tests restored the ability of gonadectomized subjects of both sexes to detect low concentrations of male urinary odors regardless of their SCG status. This suggests that T, but not its neural metabolites estradiol, or DHT, facilitates responsiveness to low concentrations of male odors in mice of both sexes. In tests given 51 days after gonadectomy without steroid treatment most male and female subjects readily detected the three highest concentrations of estrous female urinary odors whereas SCGx males and females failed to detect the lowest concentrations of these odors. After treatment with EB and then with DHT, gonadectomized mice of both sexes generally failed to detect the three lowest concentrations of estrous female urinary odors regardless of their SCG status. After T treatment; however, subjects of both sexes again detected most dilutions of estrous female urine, provided their SCG’s were intact. Again, these results suggest that T, but not estradiol or DHT, facilitates responsiveness to estrous female urinary odors. Provided such an activational effect of T is present, sympathetic, noradrenergic inputs to the MOE may enhance odorant contrast, as previously suggested [Nat. Neurosci. 2 (1999) 106], by reducing the responsiveness of olfactory neurons to low (1:120 and 1:160 dilutions) concentrations of some biologically significant odorants (e.g. male urinary odors) while facilitating their responsiveness to low to moderate (1:80 dilution) concentrations of others (e.g. estrous female urinary odors).