Efficient assembly of photosystem II in Chlamydomonas reinhardtii requires Alb3.1p, a homolog of Arabidopsis ALBINO3
Efficient assembly of photosystem II in Chlamydomonas reinhardtii requires Alb3.1p, a homolog of Arabidopsis ALBINO3
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DOI：
10.1105/tpc.023226
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发表时间：
2004-07-01
期刊：
影响因子：
11.6
通讯作者：
Eichacker, LA
中科院分区：
文献类型：
--
作者：
Ossenbühl, F;Göhre, V;Eichacker, LA
Alb3同系物Oxa1和YidC已被证明是新合成的蛋白质整合到膜中所必需的。在这里，我们表明，虽然Alb3.1p不是叶绿体编码的光系统11核心亚单位d1整合到莱茵衣藻类囊体膜中所必需的，但在alb3.1缺失突变体ac29中，d1插入到功能光系统11复合体中的过程被推迟。Alb3.1p插入膜后与D1结合，表明Alb3.1p对于光系统Ill的有效组装是必不可少的。此外，在ac29中，核编码的捕光蛋白的水平大大降低；然而，其余的天线系统仍然连接到光系统II反应中心。因此，Alb3.1p具有双重功能，是莱茵梭菌光合作用复合体中核和叶绿体编码蛋白亚基积累所必需的。
Alb3 homologs Oxa1 and YidC have been shown to be required for the integration of newly synthesized proteins into membranes. Here, we show that although Alb3.1 p is not required for integration of the plastid-encoded photosystem 11 core subunit D1 into the thylakoid membrane of Chlamydomonas reinhardtii, the insertion of D1 into functional photosystem 11 complexes is retarded in the Alb3.1 deletion mutant ac29. Alb3.1 p is associated with D1 upon its insertion into the membrane, indicating that Alb3.1 p is essential for the efficient assembly of photosystem Ill. Furthermore, levels of nucleus-encoded light-harvesting proteins are vastly reduced in ac29; however, the remaining antenna systems are still connected to photosystem II reaction centers. Thus, Alb3.1 p has a dual function and is required for the accumulation of both nucleus and plastid-encoded protein subunits in photosynthetic complexes of C. reinhardtii.