Manipulating Degradation Time in a N-isopropylacrylamide-Based Co-polymer with Hydrolysis-Dependent LCST
Manipulating Degradation Time in a N-isopropylacrylamide-Based Co-polymer with Hydrolysis-Dependent LCST
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DOI：
10.1163/156856209x451323
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发表时间：
2010-01-01
影响因子：
3.6
通讯作者：
Vernon, Brent L.
中科院分区：
文献类型：
--
作者：
Cui, Zhanwu;Lee, Bae Hoon;Vernon, Brent L.
一种热敏，生物可吸收和原位胶凝共聚物，聚(n -异丙基丙烯酰胺-co-二甲基。采用自由基共聚合的方法，以不同含量的二甲基-丙烯酸丁内酯（DBA）为原料合成了丙烯酸丁内酯。采用差示扫描量热法、凝胶渗透色谱法（结合静态光散射）、傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）和酸滴定法对材料的性质进行了表征。合成共聚物的初始低临界溶解温度（LCST）介于室温和体温之间。随着DBA含量的增加，LCST降低，但DBA侧基开环水解后LCST升高。红外光谱（FT-IR）和核磁共振光谱（NMR）显示了三种单体的共聚合，以及DBA侧基的水解依赖性开环。丙烯酸的加入提高了共聚物的初始LCST，加快了共聚物的降解速度。间接细胞毒性试验表明，这种共聚物对3T3成纤维细胞具有相对较低的细胞毒性。(C)美国内华达州，莱顿，2010
A thermosensitive, bioresorbable and in situ gelling co-polymer, poly(N-isopropylacrylamide-co-dimethyl. gamma-butyrolactone acrylate-co-acrylic acid), was synthesized by radical co-polymerization with varying dimethyl-gamma-butyrolactone acrylate (DBA) content. The materials properties were characterized using differential scanning calorimetry, gel-permeation chromatography in conjunction with static light scattering, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) and acid titration. The initial lower critical solution temperature (LCST) of the synthesized co-polymer is between room temperature and body temperature. With the increase of DBA content, the LCST decreases, but then increases after the ring-opening hydrolysis of the DBA side-group. The FT-IR and NMR spectra show the co-polymerization of three monomers, as well as the hydrolysis-dependent ring-opening of the DBA side-group. The addition of acrylic acid increases the initial LCST and accelerates the degradation rate of the co-polymer. An indirect cytotoxicity test indicated that this co-polymer has relatively low cytotoxicity as seen with 3T3 fibroblast cells. (C) Koninklijke Brill NV, Leiden, 2010