Three-dimensional organisation of mitochondrial clusters in regenerating dorsal root ganglion (DRG) neurons from neonatal rats: evidence for mobile mitochondrial pools
Three-dimensional organisation of mitochondrial clusters in regenerating dorsal root ganglion (DRG) neurons from neonatal rats: evidence for mobile mitochondrial pools
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DOI：
10.1046/j.1529-8027.2000.00153.x
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发表时间：
2000-03-01
影响因子：
3.8
通讯作者：
Roufogalis, BD
中科院分区：
文献类型：
--
作者：
Dedov, VN;Armati, PJ;Roufogalis, BD
我们首次报告了从培养的新生大鼠中分离出的正在发育的背根神经节（DRG）神经元中线粒体阵列的重排。神经元负载线粒体特异性荧光染料 JC-1，并通过共焦激光切片对新鲜神经元和培养一周的神经元进行线粒体荧光的三维 (3D) 重建。我们发现 24 小时后，线粒体会重组，在轴突小丘中形成簇。轴突延伸和神经元网络形成与线粒体簇的重新分布同时发生。在延伸的轴突中，线粒体沿轴突长度间隔开；然而，它们在分支点和生长锥中形成簇。我们的结论是，最初的线粒体簇可能是移动线粒体的存储池，能够在神经元再生和神经元生长过程中动员为轴突运输提供能量。这些发现可能与成年 DRG 神经元的轴突再生和轴突运输的速率，以及发育中的 DRG 神经元的神经元极化和轴突生长调节有关。
We report for the first time the rearrangement of mitochondrial arrays in developing dorsal root ganglion (DRG) neurons isolated from neonatal rats in culture. Neurons were loaded with the mitochondria-specific fluorescent dye JC-1, and three-dimensional (3D) reconstruction of mitochondrial fluorescence was performed by confocal laser sectioning in fresh neurons and neurons kept in culture up to a week. We found that after 24 hours the mitochondria become reorganised to form clusters in the axonal hillocks. Axonal extension and neuronal network formation coincided with a redistribution of the mitochondrial clusters. In the extended axons the mitochondria become spaced along the axonal length; however,they formed clusters in the branch points and growth cones. We conclude that the initial clusters of mitochondria may be storage pools of mobile mitochondria able to be mobilised to provide energy for axonal transport during neuronal regeneration and neuronal outgrowth. These findings may have relevance to the rate of axonal regeneration and axonal transport in adult DRG neurons, and neuronal polarisation and axonal outgrowth regulation in developing DRG neurons.