Identification and sequence analysis of prolactin receptor and its differential expression profile at various developmental stages in striped hamsters.
Identification and sequence analysis of prolactin receptor and its differential expression profile at various developmental stages in striped hamsters.
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条纹仓鼠催乳素受体的鉴定、序列分析及其在不同发育阶段的差异表达谱
DOI：
10.1590/1414-431x202010274
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Xu L
中科院分区：
文献类型：
--
作者：
Xue H;Xu J;Wu M;Chen L;Xu L
催乳素（PRL）通过与特定催乳素受体（PRLR）的结合，在生物学功能的调节中发挥重要作用。揭示PRLR在不同发育阶段的表达模式有助于更好地了解PRL在黑线仓鼠中的作用及其作用机制。本研究从成年雌性黑线仓鼠（Cricetulus barabensis）脑垂体中获得了PRLR基因的cDNA序列（2866 bp），其中包含一个834 bp的5′非翻译区（1-834 bp）、一个1848 bp的开放阅读框（835-2682 bp）和一个184 bp的3′非翻译区（2683-2866 bp）。1848个碱基对的开放阅读框架编码592个氨基酸的成熟催乳素结合蛋白。在成熟的催乳素受体中，检测到两个催乳素结合基序，12个半胱氨酸和5个潜在的天冬酰胺连接的糖基化位点。结果表明，下丘脑、垂体、卵巢和睾丸中PRLR mRNA的表达量随发育阶段的不同而不同，亚成体阶段表达量最高，老年阶段表达量最低。我们还发现PRLR mRNA在垂体中表达最高，在下丘脑中表达中等，在卵巢或睾丸中表达最低。PRLR mRNA显着高于男性比女性，除了在下丘脑和垂体7周龄条纹仓鼠。下丘脑、垂体、卵巢或睾丸中PRLR mRNA的表达与下丘脑中GnRH的表达水平呈正相关。这些结果表明，条纹仓鼠PRLR具有保守的结构域，但也具有特异性。PRLR具有多种生物学功能，包括正调节仓鼠的繁殖。
Prolactin (PRL) plays critical roles in regulation of biological functions with the binding of specific prolactin receptor (PRLR). Revealing the expression patterns of PRLR at different developmental stages is beneficial to better understand the role of PRL and its mechanism of action in striped hamsters. In this study, the cDNA sequence of PRLR (2866-base-pairs) was harvested from the pituitary of mature female striped hamsters (Cricetulus barabensis) that contains an 834-base-pair 5′-untranslated region (1-834 bp), a 1848-base-pair open reading frame (835-2682 bp), and a 184-base-pair 3′-untranslated region (2683-2866). The 1848-base-pair open reading frame encodes a mature prolactin-binding protein of 592 amino acids. In the mature PRLR, two prolactin-binding motifs, 12 cysteines, and five potential Asn-linked glycosylation sites were detected. Our results showed that the PRLR mRNA quantity in the hypothalamus, pituitary, ovaries, or testis was developmental-stage-dependent, with the highest level at sub-adult stage and the lowest level at old stage. We also found that PRLR mRNAs were highest in pituitary, medium level in hypothalamus, and lowest in ovaries or testis. PRLR mRNAs were significantly higher in males than in females, except in the hypothalamus and pituitary from 7-week-old striped hamsters. Moreover, the PRLR mRNAs in the hypothalamus, pituitary, and ovaries or testis were positively correlated with the expression levels of GnRH in the hypothalamus. These results indicated that the PRLR has conserved domain in striped hamster, but also possesses specific character. PRLR has multiple biological functions including positively regulating reproduction in the striped hamster.