Direct Transplantation of Uncultured Hair-Follicle Pluripotent Stem (hfPS) Cells Promotes the Recovery of Peripheral Nerve Injury
Direct Transplantation of Uncultured Hair-Follicle Pluripotent Stem (hfPS) Cells Promotes the Recovery of Peripheral Nerve Injury
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DOI：
10.1002/jcb.22534
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发表时间：
2010-05-01
影响因子：
4
通讯作者：
Katsuoka, Kensei
中科院分区：
文献类型：
--
作者：
Amoh, Yasuyuki;Hamada, Yuko;Katsuoka, Kensei
我们之前的研究表明，在毛囊干细胞中表达了STAR细胞标志物Nestin，这表明它们具有多能性。我们随后发现表达巢蛋白的毛囊多能干细胞(HFPS)在培养中可以分化为神经元、神经胶质细胞、角质形成细胞等细胞类型，并能促进周围神经和脊髓损伤的再生。HFPS细胞的位置被称为MPS细胞区(HfPSCA)。以前，HFPS细胞在移植到受损的神经或脊髓之前需要培养1-2个月，这对于临床应用这些细胞进行神经或脊髓修复并不是最理想的，因为患者应该在受伤后立即接受治疗。在本研究中，我们通过直接使用含有hfPSCA的毛囊上部，不经培养，将其注射到小鼠切断的坐骨神经中来解决这个问题。注射hfPSCA后，移植的HFPS细胞生长，促进了断端神经的连接。移植的HFPS细胞主要分化为神经胶质细胞，形成髓鞘，促进神经轴突生长和功能恢复。这些结果表明，将含有hfPSA的未培养毛囊上部直接移植是促进周围神经损伤修复的重要方法，具有重要的临床应用价值。J.细胞。生物化学。110：272-277,2010。(C)2010年Wiley-Liss公司
We previously showed that the stern cell marker nestin is expressed in hair follicle stem cells which suggested their pluripotency. We subsequently showed that the nestin-expressing hair-follicle pluripotent stem (hfPS) cells can differentiate in culture to neurons, glial cells, keratinocytes, and other cell types and can promote regeneration of peripheral nerve and spinal cord injuries upon injection to the injured nerve or spinal cord. The location of the hfPS cells has been termed the MPS cell area (hfPSCA). Previously, hfPS cells were cultured for 1-2 months before transplantation to the injured nerve or spinal cord which would not be optimal for clinical application of these cells for nerve or spinal cord repair, since the patient should be treated soon after injury. In the present study, we addressed this issue by directly using the upper part of the hair follicle containing the hfPSCA, without culture, for injection into the severed sciatic nerve in mice. After injection of hfPSCA, the implanted hfPS cells grew and promoted joining of the severed nerve. The transplanted hfPS cells differentiated mostly to glial cells forming myelin sheaths, which promoted axonal growth and functional recovery of the severed nerve. These results suggest that the direct transplantation of the uncultured upper part of the hair follicle containing the hfPSA is an important method to promote the recovery of peripheral nerve injuries and has significant clinical potential. J. Cell. Biochem. 110: 272-277, 2010. (C) 2010 Wiley-Liss, Inc.