A Survey of the Occurrence of Fusarium Mycotoxins in Biscuits in Japan by Using LC/MS
A Survey of the Occurrence of Fusarium Mycotoxins in Biscuits in Japan by Using LC/MS
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DOI：
10.1248/jhs.56.188
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发表时间：
2010-04
影响因子：
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通讯作者：
Hiroki Tanaka;Y. Sugita‐Konishi;M. Takino;Toshitsugu Tanaka;A. Toriba;K. Hayakawa
中科院分区：
文献类型：
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作者：
Hiroki Tanaka;Y. Sugita‐Konishi;M. Takino;Toshitsugu Tanaka;A. Toriba;K. Hayakawa
采用一种快速、灵敏的同时检测雪腐镰刀菌烯醇（NIV）、脱氧雪腐镰刀菌烯醇（DON）、镰刀菌酮-X（FX）、3-乙酰脱氧雪腐镰刀菌烯醇（3ADON）、HT-2毒素（HT-2）、T-2毒素（T-2）和玉米赤霉烯酮（ZEN）的方法，对日本201份小麦饼干中这些真菌毒素的自然存在情况进行了调查。通过LC/MS与大气压光电离（APPI）分析样品。通过液相色谱/飞行时间质谱（LC/TOFMS）进行进一步确认。NIV、DON、HT-2、T-2和ZEN每种镰刀菌毒素的平均污染分别为3.1、23、0.7、0.1和4.2 ng/g。在120个样本中观察到多种毒素，但未检测到FX和3ADON。NIV、DON、HT-2、T-2和ZEN的这些毒素的发生率分别为41%、98%、19%、11%和2%。普通小麦饼干与婴儿小麦饼干的浓度和发病率无显著差异。这是日本首次报道饼干中存在NIV、DON、HT-2、T-2和ZEN。
By adopting a rapid and sensitive method for simultaneous detection of nivalenol (NIV), deoxynivalenol (DON), fusarenon-X (FX), 3-acetyl deoxinivalenol (3ADON), HT-2 toxin (HT-2), T-2 toxin (T-2) and zearalenone (ZEN), the natural occurrence of these mycotoxins in biscuits made of wheat (201 samples) in Japan was surveyed. Samples were analyzed by LC/MS with atmospheric pressure photo ionization (APPI). Further confirmation was performed by liquid chromatography/time of flight mass spectrometry (LC/TOFMS). The average contamination of each Fusarium mycotoxin was 3.1, 23, 0.7, 0.1 and 4.2 ng/g for NIV, DON, HT-2, T-2 and ZEN, respectively. Multiple toxins were observed in 120 samples while FX and 3ADON were not detected. The incidence of these toxins was 41% for NIV, 98% for DON, 19% for HT-2, 11% for T-2 and 2% for ZEN. There were no significant differences in the concentration and incidence between conventional biscuits made of wheat and biscuits made of wheat for infants. This is the first report concerning the presence of NIV, DON, HT-2, T-2 and ZEN in biscuits in Japan.