Endothelin-1 of canine basilar artery in vasospasm.
Endothelin-1 of canine basilar artery in vasospasm.
复制标题
血管痉挛时犬基底动脉的内皮素-1。
DOI：
--
复制
发表时间：
1992
影响因子：
4.1
通讯作者：
Hideki Shindo
中科院分区：
文献类型：
--
作者：
I. Yamaura;E. Tani;Y. Maeda;N. Minami;Hideki Shindo
采用两次出血法诱导成年杂种犬脑血管痉挛。小心去除周围的血凝块后，将基底动脉快速冷冻，并通过夹心酶免疫测定法测量其免疫反应性内皮素-1（内皮细胞和血管平滑肌细胞产生的强血管收缩剂）的水平。血管痉挛前的免疫反应性内皮素-1水平（平均值+/-标准差）为112.9 +/- 7.0 pg/mg蛋白质，血管痉挛后第2天为180.4 +/- 24.7 pg/mg蛋白质，第7天为115.0 +/- 24.0 pg/mg蛋白质，显示免疫反应性显着增加（p小于0.01）仅在第2天出现内皮素-1。此外，第2天局部应用抗内皮素-1单克隆抗体可中度逆转血管痉挛，但在第7天对局部应用单克隆抗体具有抵抗作用。提示内皮素-1可以在脑血管痉挛的早期阶段起触发作用，但后期脑血管痉挛的维持可能独立于内皮素-1。
Cerebral vasospasm was induced in adult mongrel dogs by a two-hemorrhage method. The basilar arteries were quickly frozen after careful removal of surrounding blood clot and their level of immunoreactive endothelin-1, a strong vasoconstrictor produced by the endothelial and vascular smooth-muscle cells, was measured by sandwich-enzyme immunoassay. The levels of immunoreactive endothelin-1 (mean +/- standard deviation) were 112.9 +/- 7.0 pg/mg protein prior to vasospasm, 180.4 +/- 24.7 pg/mg protein on Day 2 after vasospasm, and 115.0 +/- 24.0 pg/mg protein on Day 7, showing a significant increase (p less than 0.01) in immunoreactive endothelin-1 only on Day 2. In addition, vasospasm was moderately reversed by the topical application of monoclonal antibody against endothelin-1 on Day 2 but rather resistant to topical monoclonal antibody on Day 7. It is suggested that endothelin-1 could act as a trigger in the early stages of cerebral vasospasm, but that the maintenance of cerebral vasospasm at later stages might be independent of endothelin-1.
内皮素作用：蛋白激酶 C 抑制剂的抑制和磷酸肌醇的参与。
DOI：
10.1016/s0006-291x(89)80193-7
发表时间：
1989
影响因子：
3.1
作者：
Sugiura,M;Inagami,T;Hare,GM;Johns,JA
通讯作者：
Johns,JA
内皮素是成纤维细胞和平滑肌细胞的一种有效的新型激动剂，刺激磷脂酰肌醇水解、二酰甘油释放和基因表达。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Muldoon,LL;Rodland,KD;Forsythe,ML;Magun,BE
通讯作者：
Magun,BE
通过血管紧张素转换酶抑制逆转实验性迟发性脑血管痉挛。
影响因子：
4.1
作者：
Gavras,H;Andrews,P;Papadakis,N
通讯作者：
Papadakis,N