Transient appearance of Ca(2+) -permeable AMPA receptors is crucial for the production of repetitive LTP-induced synaptic enhancement (RISE) in cultured hippocampal slices.
Transient appearance of Ca(2+) -permeable AMPA receptors is crucial for the production of repetitive LTP-induced synaptic enhancement (RISE) in cultured hippocampal slices.
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Ca(2) 渗透性 AMPA 受体的短暂出现对于培养海马切片中重复 LTP 诱导的突触增强 (RISE) 的产生至关重要。
DOI：
10.1002/hipo.23206
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Ogura A.
中科院分区：
文献类型：
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作者：
Tominaga-Yoshino K;Urakubo T;Ueno Y;Kawaai K;Saito S;Tashiro T;Ogura A.
我们之前已经证明，在大鼠海马脑片培养物中，谷氨酸重复诱导长时程增强（LTP）（100 μM，3 min，间隔24 h三次）引起了持久的突触增强，伴随着突触发生，这种现象称为RISE（重复LTP诱导的突触增强）。在这里，我们研究了Ca 2+渗透性（CP）AMPA受体（AMPAR）在RISE建立中的作用。我们首先在刺激后2-3天从CA 3-CA 1突触记录的场EPSP中发现了对Joro‐spider toxin（JSTX）敏感的组分，JSTX是CP-AMPAR的阻断剂，但在9-10天内没有发现该组分。我们还观察到AMPAR介导的电流整流仅在刺激后2-3天出现，使用来自CA 1锥体神经元的全细胞膜片钳记录。这些发现表明，CP-AMPAR在RISE的发育阶段瞬时表达。在这个发育阶段，JSTX阻断CP-AMPAR 24小时，抑制了RISE的建立，伴随着超微结构水平上小突触的丢失。这些结果表明，瞬时诱导的CP-AMPAR在持久海马突触可塑性（RISE）的发育阶段的突触发生中起关键作用。
We have previously shown that repetitive induction of long‐term potentiation (LTP) by glutamate (100 μM, 3 min, three times at 24‐hr intervals) provoked long‐lasting synaptic enhancement accompanied by synaptogenesis in rat hippocampal slice cultures, a phenomenon termed RISE (repetitive LTP‐induced synaptic enhancement). Here, we examined the role of Ca2+‐permeable (CP) AMPA receptors (AMPARs) in the establishment of RISE. We first found a component sensitive to the Joro‐spider toxin (JSTX), a blocker of CP‐AMPARs, in a field EPSP recorded from CA3‐CA1 synapses at 2–3 days after stimulation, but this component was not found for 9–10 days. We also observed that rectification of AMPAR‐mediated current appeared only 2–3 days after stimulation, using a whole‐cell patch clamp recording from CA1 pyramidal neurons. These findings indicate that CP‐AMPAR is transiently expressed in the developing phase of RISE. The blockade of CP‐AMPARs by JSTX for 24 hr at this developing phase inhibited RISE establishment, accompanied by the loss of small synapses at the ultrastructural level. These results suggest that transiently induced CP‐AMPARs play a critical role in synaptogenesis in the developing phase of long‐lasting hippocampal synaptic plasticity, RISE.