Genetic control of the immune response to staphylococcal nuclease. IV. H-2-linked control of the relative proportions of antibodies produced to different determinants of native nuclease.
Genetic control of the immune response to staphylococcal nuclease. IV. H-2-linked control of the relative proportions of antibodies produced to different determinants of native nuclease.
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DOI：
10.1084/jem.145.1.123
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
Sachs DH
中科院分区：
文献类型：
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作者：
Berzofsky JA;Schechter AN;Shearer GM;Sachs DH
已在几种小鼠品系中研究了抗天然葡萄球菌核酸酶的抗血清中不同特异性抗体的相对比例，其中抗体反应已被证明是在H-2-连接的Ir基因控制下。开发了一种方法，其中针对增加的片段浓度滴定与不同放射性标记的核酸酶片段的结合，直到抗血清对该片段的结合能力饱和。在比较低应答（H-2b）菌株C57 BL/10与其同源高应答对应物B10.A（H-2a）时，发现这两个菌株针对天然核酸酶上的不同决定簇产生显著且可重复的不同比例的抗体。由于这两种菌株仅在H-2处不同，因此具有相同的免疫球蛋白结构基因库，我们得出结论，H-2连接的Ir基因可以彼此独立地控制对相同抗原分子上的不同决定簇的反应。这一结果表明H-2连锁基因在选择特异性B细胞中的可能作用。
The relative proportions of antibodies of different specificities within antisera raised to native staphylococcal nuclease have been studied in several strains of mice in which the antibody response has been shown to be under H-2-linked Ir-gene control. A method was developed in which binding to different radiolabeled fragments of nuclease was titrated against increasing fragment concentration until the binding capacity of the antiserum for that fragment was saturated. In comparing the low responder (H-2b) strain C57BL/10 with its congenic high responder counterpart B10.A (H-2a), it was found that the two strains made markedly and reproducibly different proportions of antibodies to different determinants on native nuclease. Since these two strains differ only at H-2, and therefore have identical immunoglobulin structural gene repertoires, we conclude that H-2-linked Ir genes can control the response to different determinants on the same antigen molecule independently of one another. This result suggests a possible role of H-2-linked genes in the selection of specific B cells.