The Intertidal Harpacticoid Copepod Tigriopus japonicus (Crustacea: Copepoda) β-actin Gene: Cloning, Sequence and Intraspecies Variation
The Intertidal Harpacticoid Copepod Tigriopus japonicus (Crustacea: Copepoda) β-actin Gene: Cloning, Sequence and Intraspecies Variation
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DOI：
10.1080/1085566031000141135
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发表时间：
2003-07
期刊：
影响因子：
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通讯作者：
Il-Chan Kim;Y. J. Kim;Sung Joon Song;Jae-seong Lee;Wonchoel Lee
中科院分区：
文献类型：
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作者：
Il-Chan Kim;Y. J. Kim;Sung Joon Song;Jae-seong Lee;Wonchoel Lee
利用聚合酶链式反应从日本虎虎的基因组中扩增出β-肌动蛋白基因，并将其克隆到pCRII载体中。日本血吸虫β-肌动蛋白基因的几个克隆全长1662-1676bp，内含子3或4有部分碱基的增减，但它们一般由5个外显子和4个内含子组成，具有很高的同源性，表明该基因具有多态性。外显子和内含子边界符合GT/AG规则。日本血吸虫β-肌动蛋白基因与鱼类和人类基因在氨基酸水平上的同源性分别为99.2%和98.4%。对日本血吸虫β-肌动蛋白基因的系统发育意义进行了讨论。
The Tigriopus japonicus β-actin genes were amplified from genomic DNA by polymerase chain reaction (PCR) and cloned into pCRII vector. Several clones of the T. japonicus β-actin gene spanned 1662–1676 bp with gains or losses of some bases in intron 3 or 4 but they consisted generally of 5 exons and 4 introns with very high homology, implying polymorphism of this gene. The exon and intron boundaries were matched with the GT/AG rule. The T. japonicus β-actin gene showed high homology to the fish (Rivulus marmoratus) and human genes, 99.2 and 98.4%, respectively at the amino acid sequence level. The phylogenetic implications inferred from the T. japoncius β-actin gene are discussed.