Selective excitation of tryptophan fluorescence decay in proteins using a subnanosecond 295 nm light-emitting diode and time-correlated single-photon counting
Selective excitation of tryptophan fluorescence decay in proteins using a subnanosecond 295 nm light-emitting diode and time-correlated single-photon counting
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DOI：
10.1063/1.1984088
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发表时间：
2005-06-27
影响因子：
4
通讯作者：
Birch, DJS
中科院分区：
文献类型：
--
作者：
McGuinness, CD;Sagoo, K;Birch, DJS
我们展示了一个AlGaN发光二极管（LED）给出的脉冲类似于600 ps的半高全宽，0.35 μ W的平均功率，0.6 mW的峰值功率，和类似于12 nm的带宽在295 nm。该光源是蛋白质内在色氨酸荧光衰减研究的理想选择，无需不必要的酪氨酸激发，并为使用荧光衰减时间的芯片实验室蛋白质测定铺平了道路。人血清白蛋白的荧光衰减和各向异性衰减测量的报告和有用的295 nm的LED显示在纳秒闪光灯和LED的标称波长为280 nm的发射相比。
We demonstrate an AlGaN light-emitting diode (LED) giving pulses of similar to 600 ps full width half maximum, 0.35 mu W average power, 0.6 mW peak power, and similar to 12 nm bandwidth at 295 nm. This source is ideal for protein intrinsic tryptophan fluorescence decay research without the unwanted excitation of tyrosine and paves the way to lab-on-a-chip protein assays using fluorescence decay times. Fluorescence decay and anisotropy decay measurements of human serum albumin are reported and the usefulness of the 295 nm LED demonstrated in comparisons with a nanosecond flashlamp and LEDs with nominal wavelength emission of 280 nm.