Database-driven multi locus sequence typing (MLST) of bacterial pathogens
Database-driven multi locus sequence typing (MLST) of bacterial pathogens
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DOI：
10.1093/bioinformatics/17.11.1077
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发表时间：
2001-11-01
期刊：
影响因子：
5.8
通讯作者：
Spratt, BG
中科院分区：
文献类型：
--
作者：
Chan, MS;Maiden, MCJ;Spratt, BG
动机：多位点序列分型（Multi - Locus Sequence Typing， MLST）是一种基于7个管家基因内部片段序列测定的细菌分型新方法。它已被证明在表征和监测致病和耐抗生素细菌谱系方面是有用的。这种方法的优点在于，与使用大多数其他类型方法获得的数据不同，序列数据是明确的，可以保存在中央数据库中，并通过web服务器进行查询。结果：已经开发了一个数据库驱动的软件系统（mlstdb），供全球的公共卫生实验室和研究人员使用，通过互联网向中央持有的数据库查询其核苷酸序列数据。mlstdb系统由一组perl脚本组成，用于定义数据库表、生成数据库管理接口、查询数据库管理接口的动态web页面和查询数据库的动态web页面。
Motivation: Multi Locus Sequence Typing (MLST) is a newly developed typing method for bacteria based on the sequence determination of internal fragments of seven house-keeping genes. It has proved useful in characterizing and monitoring disease-causing and antibiotic resistant lineages of bacteria. The strength of this approach is that unlike data obtained using most other typing methods, sequence data are unambiguous, can be held on a central database and be queried through a web server.Results: A database-driven software system (mlstdb) has been developed, which is used by public health laboratories and researchers globally to query their nucleotide sequence data against centrally held databases over the internet. The mlstdb system consists of a set of perl scripts for defining the database tables and generating the database management interface and dynamic web pages for querying the database management interface and dynamic web pages for querying the databases.