Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan
Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan
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DOI：
10.1038/s41598-019-49541-y
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发表时间：
2019-09-11
影响因子：
4.6
通讯作者：
Li, Tian-Cheng
中科院分区：
文献类型：
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作者：
Zhou, Xianfeng;Bai, Huimin;Li, Tian-Cheng
新泽西多瘤病毒（NJPyV）于2014年在一名胰腺移植受者的血管内皮细胞中被发现。本文在重组杆状病毒系统中，处理了在昆虫细胞中表达的NJPyV的VP1蛋白。该蛋白以细胞类型依赖的方式自组装成病毒样颗粒（NJPyV-LPs），然后将颗粒释放到培养基中。球形50-nm-dia。从表达vp1的Sf9细胞的上清液中以1.33 g/cm(3)的速度纯化出大小均匀、形态与多瘤病毒原生颗粒相似的NJPyV-LPs。我们研究了纯化的NJPyV - lps的抗原特性，并进行了基于vlp的酶免疫测定，以确定1-70岁日本普通人群中NJPyV感染的年龄特异性患病率。抗njpyv抗体血清总阳性率仅为1.8%。这可能与NJPyV在日本的低环流有关。这是在亚洲（n = 1,050）进行的首例大规模NJPyV血清学调查报告。
New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient's vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self-assembled into virus-like particles (NJPyV-LPs) in a cell-type-dependent manner, and the particles were then released into the culture media. Spherical -50-nm-dia. NJPyV-LPs of uniform size with morphology resembling that of the native particles of polyomaviruses were purified from the fraction at 1.33 g/cm(3) in supernatants of VP1-expressing Sf9 cells. We investigated the antigenic properties of purified NJ PyV-LPs and performed a VLP-based enzyme immunoassay to determine the age-specific prevalence of NJPyV infection in a general Japanese population aged 1-70 years. The overall seropositivity rate of anti-NJPyV antibodies was only 1.8%. This might be explained by the low circulation of NJPyV in Japan. This is the first report of a large-scale serological survey of NJPyV in Asia (n = 1,050).