Titanium surface characteristics, including topography and wettability, alter macrophage activation.
Titanium surface characteristics, including topography and wettability, alter macrophage activation.
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DOI：
10.1016/j.actbio.2015.12.003
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发表时间：
2016-02
影响因子：
9.7
通讯作者：
Olivares-Navarrete R
中科院分区：
文献类型：
--
作者：
Hotchkiss KM;Reddy GB;Hyzy SL;Schwartz Z;Boyan BD;Olivares-Navarrete R
生物材料的表面特性包括化学、地形和润湿性调节细胞反应。先前的研究表明，增加金属骨科和牙科种植体的表面粗糙度可以改善种植体周围的骨形成。关于种植体表面特性如何影响产生伤口愈合微环境的免疫细胞，我们知之甚少。我们研究的目的是研究表面修饰对巨噬细胞活化和细胞因子产生的影响。巨噬细胞在7个表面培养：组织培养聚苯乙烯（TCPS）对照；疏水和亲水光滑Ti (PT和氧等离子体处理（plasma） PT)；疏水和亲水微孔Ti (SLA和等离子体SLA)，疏水和亲水纳米和微粗糙Ti（老化的modSLA和modSLA）。光滑钛诱导炎性巨噬细胞（m1样）激活，如白细胞介素IL-1β、IL-6和TNFα水平升高所示。相反，亲水粗钛诱导巨噬细胞激活，类似于抗炎的m2样状态，增加白细胞介素IL-4和IL-10的水平。这些结果表明，在高表面润湿性材料上培养的巨噬细胞可产生抗炎微环境，这一特性可用于改善生物材料的愈合反应。
Biomaterial surface properties including chemistry, topography, and wettability regulate cell response. Previous studies have shown that increasing surface roughness of metallic orthopaedic and dental implants improved bone formation around the implant. Little is known about how implant surface properties can affect immune cells that generate a wound healing microenvironment. The aim of our study was to examine the effect of surface modifications on macrophage activation and cytokine production. Macrophages were cultured on seven surfaces: tissue culture polystyrene (TCPS) control; hydrophobic and hydrophilic smooth Ti (PT and oxygen-plasma-treated (plasma) PT); hydrophobic and hydrophilic microrough Ti (SLA and plasma SLA), and hydrophobic and hydrophilic nano-and micro-rough Ti (aged modSLA and modSLA). Smooth Ti induced inflammatory macrophage (M1-like) activation, as indicated by increased levels of interleukins IL-1β, IL-6, and TNFα. In contrast, hydrophilic rough titanium induced macrophage activation similar to the anti-inflammatory M2-like state, increasing levels of interleukins IL-4 and IL-10. These results demonstrate that macrophages cultured on high surface wettability materials produce an anti-inflammatory microenvironment, and this property may be used to improve the healing response to biomaterials.