Molecular linkage mapping and phylogeny of the chalcone synthase multigene family in soybean
Molecular linkage mapping and phylogeny of the chalcone synthase multigene family in soybean
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DOI：
10.1007/s00122-005-1950-7
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发表时间：
2005-05-01
影响因子：
5.4
通讯作者：
Takahashi, R
中科院分区：
文献类型：
--
作者：
Matsumura, H;Watanabe, S;Takahashi, R
查尔酮合成酶（Chalcone synthase，CHS）是大豆类黄酮合成途径中的关键酶，由CHS 1-CHS 8和dCHS 1等多基因家族编码。CHS 1、CHS 3和CHS 4的串联重复以及被认为位于附近的dCHS 1包含抑制种皮着色的I基因座。本研究旨在通过PCR技术对所有CHS成员进行定位。根据两个品种（具有黄色种皮的Misuzudaizu（II）和具有棕色种皮的Moshidou Gong 503（ii）的5 '上游区域或第一内含子的核苷酸序列的品种差异构建引物。利用150个重组自交系和360个标记进行了遗传连锁分析。连锁图谱分析证实，CHS 1、CHS 3、CHS 4、dCHS 1和I位点位于分子连锁群（MLG）A2的相同位置。CHS 5定位在距离基因簇0.3cM处。CHS 2和CHS 6分别位于MLG A1和K的中间区域，而CHS 7和CHS 8分别位于MLG D1 a和B1的远端。系统发育分析表明，CHS 1，CHS 3，CHS 4和CHS 5是密切相关的，这表明基因重复可能发生了重复，形成I位点。此外，位于远端的CHS 7和CHS 8以及位于MLG中部周围的I位点周围的CHS 2、CHS 6和CHS成员也相关。古老的四倍化和重复复制可能是大豆CHS多基因家族复杂遗传位点进化的原因。
Chalcone synthase (CHS), the key enzyme in the flavonoid biosynthesis pathway, is encoded by a multigene family, CHS1 - CHS8 and dCHS1 in soybean. A tandem repeat of CHS1, CHS3 and CHS4, and dCHS1 that is believed to be located in the vicinity comprises the I locus that suppresses coloration of the seed coat. This study was conducted to determine the location of all CHS members by using PCR-based DNA markers. Primers were constructed based on varietal differences in either the nucleotide sequence of the 5'-upstream region or the first intron of two cultivars, Misuzudaizu, with a yellow seed coat (II), and Moshidou Gong 503, with a brown seed coat (ii). One hundred and fifty recombinant inbred lines that originated from a cross between these two cultivars were used for linkage mapping together with 360 markers. Linkage mapping confirmed that CHS1, CHS3, CHS4, dCHS1, and the I locus are located at the same position in molecular linkage group (MLG) A2. CHS5 was mapped at a distance of 0.3 cM from the gene cluster. CHS2 and CHS6 were located in the middle region of MLGs A1 and K, respectively, while CHS7 and CHS8 were found at the distal end of MLGs D1a and B1, respectively. Phylogenetic analysis indicated that CHS1, CHS3, CHS4, and CHS5 are closely related, suggesting that gene duplication may have occurred repeatedly to form the I locus. In addition, CHS7 and CHS8 located at the distal end and CHS2, CHS6, and CHS members around the I locus located around the middle of the MLG are also related. Ancient tetraploidization and repeated duplication may be responsible for the evolution of the complex genetic loci of the CHS multigene family in soybean.