Blood glutathione concentrations in a large-scale human study.
Blood glutathione concentrations in a large-scale human study.
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DOI：
10.1093/clinchem/42.1.64
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发表时间：
1996
影响因子：
9.3
通讯作者：
J. Richie;L. Skowroński;P. Abraham;Y. Leutzinger
中科院分区：
文献类型：
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作者：
J. Richie;L. Skowroński;P. Abraham;Y. Leutzinger
人们对人体血液中谷胱甘肽(GSH)的变异性知之甚少。因此，我们希望开发和验证一种快速测量全血中GSH浓度的方法，并应用该方法在一项大规模的自由生活成年人研究中确定GSH的变化。该方法灵敏度高(检出限<lt；5pmol)，精密度高，批间变异系数为2.3%，采样变异系数为3.6%。应用此法对715名成人进行筛查，观察到谷胱甘肽浓度的三倍范围，平均值为1.02 mmol/L，变异系数为17%。吸烟者的谷胱甘肽浓度比不吸烟者高8-10%(P<；0.001)。虽然我们没有观察到GSH的性别差异，但在55岁的女性中，GSH/血红蛋白比率比年龄较大的受试者高8-18%(P<；0.05)。本文提供的结果验证了这种方法在大规模人体研究中的使用，并提供了有关成人血液GSH变化和正常值的信息。
Little is known about the variability of blood glutathione (GSH) in human subjects. Thus, we wanted to develop and validate a rapid method for measuring GSH concentrations in whole blood and apply this method to ascertain the variation of GSH in a large-scale study of free-living adults. The assay was highly sensitive (detection limit < 5 pmol) and precise, with an interassay variation of 2.3% and a sampling variation of 3.6%. Applying this method to screen 715 adults, we observed a threefold range of GSH concentrations, with a mean of 1.02 mmol/L and CV of 17%. GSH concentrations were 8-10% greater in smokers than in nonsmokers (P < 0.001). Although we observed no sex differences for GSH, GSH/hemoglobin ratios were 8-18% greater in women < 55 years old than in older subjects (P < 0.05). The results presented here validate the use of this method for large-scale human studies and provide information on the variation and normal values of blood GSH in adults.