Initiation of programmed cell death in the suspensor is predominantly regulated maternally in a tobacco hybrid.
Initiation of programmed cell death in the suspensor is predominantly regulated maternally in a tobacco hybrid.
复制标题
在烟草杂交种中，悬柄中程序性细胞死亡的启动主要由母体调节。
DOI：
10.1038/srep29467
复制
发表时间：
2016-07-19
影响因子：
4.6
通讯作者：
Sun MX
中科院分区：
文献类型：
--
作者：
Luo A;Zhao P;Zhang LY;Sun MX
在动物胚胎基因组激活之前，沉积在卵中的母体基因产物调节早期胚胎发生。而在高等植物中，据信亲本来源的基因有助于早期胚胎发生。然而，很少有人知道有关的特定过程中，父母起源的基因参与早期胚胎发生。以前，我们发现，启动程序性细胞死亡（PCD）的胚柄是由半胱氨酸蛋白酶抑制剂，NtCYS。在这里，我们证实，父母的转录有助于PCD，但母亲的NtCYS等位基因的相对表达水平远高于父亲的等位基因在烟草种间杂种早期胚胎。母本NtCYS等位基因的表达水平明显降低，这是PCD启动所必需的，而父本NtCYS等位基因的表达水平无明显变化。有趣的是，杂种胚柄的PCD模式和杂种胚柄的形态与母本相似。我们的研究结果表明，NtCYS介导的PCD启动在杂种胚柄可能是控制在一个母亲的显性方式。这一发现代表了一个例子，在早期胚胎发生过程中的一个特定的发育事件的父母的成绩单的参与。
Maternal gene products deposited in the egg regulate early embryogenesis before activation of the embryonic genome in animals. While in higher plants, it is believed that genes of parental origin contribute to early embryogenesis. However, little is known regarding the particular processes in which genes of parental origin are involved during early embryogenesis. Previously, we found that the initiation of programmed cell death (PCD) in the suspensor of the embryo is regulated by the cystatin, NtCYS. Here, we confirmed that both parental transcripts contribute to PCD, but the relative expression level of the maternal NtCYS allele was much higher than that of the paternal allele in early embryos of tobacco interspecific hybrids. The expression level of the maternal NtCYS allele was decreased markedly, which was necessary for the initiation of PCD, while the paternal allele didn’t change. Interestingly, the pattern of PCD in the hybrid suspensor and the morphology of the hybrid suspensor were similar to those of the maternal parent. Our results suggest that NtCYS-mediated PCD initiation in the hybrid suspensor is likely controlled in a maternal dominant manner. This finding represents an example of the involvement of parental transcripts in a specific developmental event during early embryogenesis.