Probable Pangolin Origin of SARS-CoV-2 Associated with the COVID-19 Outbreak
Probable Pangolin Origin of SARS-CoV-2 Associated with the COVID-19 Outbreak
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DOI：
10.1016/j.cub.2020.03.022
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发表时间：
2020-04-06
期刊：
影响因子：
9.2
通讯作者：
Zhang, Zhigang
中科院分区：
文献类型：
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作者：
Zhang, Tao;Wu, Qunfu;Zhang, Zhigang
由2019年新型冠状病毒（SARS-CoV-2）引起的2019冠状病毒病（COVID-19）疫情始于中国武汉市，并在全球范围内广泛传播。当前，探索潜在的SARS-CoV-2中间宿主是控制COVID-19传播的关键。因此，我们重新研究了Liu等人[b]从已发表的穿山甲肺样本中检测到sars - cov样冠状病毒的数据。我们在死亡的马来亚穿山甲中发现了sars -CoV-2样冠状病毒（命名为穿山甲冠状病毒）的基因组和进化证据。在全基因组水平上，穿山甲冠状病毒与SARS-CoV-2和BatCoV RaTG13的相似性分别为91.02%和90.55%。除了RaTG13外，穿山甲冠状病毒是与SARS-CoV-2关系最密切的冠状病毒。穿山甲冠状病毒S1蛋白与SARS-CoV-2的相关性远高于与RaTG13的相关性。与人类ACE2相互作用的5个关键氨基酸残基在穿山甲cov和SARS-CoV-2之间完全一致，但在RaTG13中存在4个氨基酸突变。在SARS-CoV-2中，穿山甲- cov和RaTG13在S1/S2切割位点都丢失了推测的furin识别序列基序。总之，本研究表明穿山甲物种是sars - cov -2样冠状病毒的天然储存库。
An outbreak of coronavirus disease 2019 (COVID-19) caused by the 2019 novel coronavirus (SARS-CoV-2) began in the city of Wuhan in China and has widely spread worldwide. Currently, it is vital to explore potential intermediate hosts of SARS-CoV-2 to control COVID-19 spread. Therefore, we reinvestigated published data from pangolin lung samples from which SARS-CoV-like CoVs were detected by Liu et al. [1]. We found genomic and evolutionary evidence of the occurrence of a SARS-CoV-2-like CoV (named Pangolin-CoV) in dead Malayan pangolins. Pangolin-CoV is 91.02% and 90.55% identical to SARS-CoV-2 and BatCoV RaTG13, respectively, at the whole-genome level. Aside from RaTG13, Pangolin-CoV is the most closely related CoV to SARS-CoV-2. The S1 protein of Pangolin-CoV is much more closely related to SARS-CoV-2 than to RaTG13. Five key amino acid residues involved in the interaction with human ACE2 are completely consistent between Pangolin-CoV and SARS-CoV-2, but four amino acid mutations are present in RaTG13. Both Pangolin-CoV and RaTG13 lost the putative furin recognition sequence motif at S1/S2 cleavage site that can be observed in the SARS-CoV-2. Conclusively, this study suggests that pangolin species are a natural reservoir of SARS-CoV-2-like CoVs.