CCC1 is a transporter that mediates vacuolar iron storage in yeast
CCC1 is a transporter that mediates vacuolar iron storage in yeast
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DOI：
10.1074/jbc.m103944200
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发表时间：
2001-08-03
影响因子：
4.8
通讯作者：
Kaplan, J
中科院分区：
文献类型：
--
作者：
Li, LT;Chen, OS;Kaplan, J
发芽酵母酿酒酵母在没有外源铁的情况下可以世代生长，这表明它具有储存细胞内铁的能力。由于细胞可以通过内吞作用积累铁，我们研究了内吞作用缺陷的酵母中的铁代谢。我们证明了内吞缺陷酵母(Delta End4)可以将铁储存在液泡中，这表明铁从细胞质转移到液泡。使用几种不同的标准，我们证明了CCC1编码一个转运蛋白，影响液泡中铁和Mn2+的积累。定位于液泡的CCC1的过表达降低了细胞内的铁含量，增加了液泡铁的含量。相反，CCc1的缺失会导致液泡铁含量降低和铁储存减少，从而影响细胞内铁水平和细胞生长。此外，Delta ccc1细胞对外部铁的敏感性增加。铁转运蛋白FET4的异位表达加剧了对铁的敏感性。这些结果表明，酵母可以将铁储存在液泡中，CCC1参与了铁从细胞质到液泡的转移。
The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that endocytosis-defective yeast (Delta end4) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn2+ in vacuoles. Overexpression of CCC1, which is localized to the vacuole, lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth. Furthermore Delta ccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron transporter FET4. These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole.