Recognition of pre- and postsynaptic neurons via nephrin/NEPH1 homologs is a basis for the formation of the Drosophila retinotopic map
Recognition of pre- and postsynaptic neurons via nephrin/NEPH1 homologs is a basis for the formation of the Drosophila retinotopic map
复制标题
DOI：
10.1242/dev.047332
复制
发表时间：
2010-10
期刊：
影响因子：
4.6
通讯作者：
Atsushi Sugie;Daiki Umetsu;Tetsuo Yasugi;K. Fischbach;T. Tabata
中科院分区：
文献类型：
--
作者：
Atsushi Sugie;Daiki Umetsu;Tetsuo Yasugi;K. Fischbach;T. Tabata
在神经系统的许多部位都发现了地形图，它维持了神经元在轴突连接顺序中的空间顺序。在这里，我们将重点放在果蝇视觉系统第一神经节中视网膜轴突与其突触后伙伴（层状神经元）之间的通信上，作为地形图形成的模型。有丝分裂后的板前体细胞在接受新到达的视网膜轴突传递的Hedgehog信号后分化，在成熟到延伸神经突之前，向视网膜轴突延伸短过程，形成板柱。层状柱为视网膜轴突和层状神经元之间建立刻板突触提供了细胞基础。在这项研究中，我们鉴定了两种细胞粘附分子：Hibris，在有丝分裂后的板前体细胞中表达；和Roughest，在视网膜轴突上表达。这两种蛋白都属于nephrin/NEPH1家族。我们提供的证据表明，有丝分裂后板前体细胞和视网膜轴突之间的识别是由Hibris和Roughest之间的相互作用介导的。这些发现揭示了突触前神经元轴突传递信号诱导目标区域突触后伙伴发育的机制。然后突触后伙伴识别突触前轴突形成集合，从而沿前/后轴建立地形图。
Topographic maps, which maintain the spatial order of neurons in the order of their axonal connections, are found in many parts of the nervous system. Here, we focus on the communication between retinal axons and their postsynaptic partners, lamina neurons, in the first ganglion of the Drosophila visual system, as a model for the formation of topographic maps. Post-mitotic lamina precursor cells differentiate upon receiving Hedgehog signals delivered through newly arriving retinal axons and, before maturing to extend neurites, extend short processes toward retinal axons to create the lamina column. The lamina column provides the cellular basis for establishing stereotypic synapses between retinal axons and lamina neurons. In this study, we identified two cell-adhesion molecules: Hibris, which is expressed in post-mitotic lamina precursor cells; and Roughest, which is expressed on retinal axons. Both proteins belong to the nephrin/NEPH1 family. We provide evidence that recognition between post-mitotic lamina precursor cells and retinal axons is mediated by interactions between Hibris and Roughest. These findings revealed mechanisms by which axons of presynaptic neurons deliver signals to induce the development of postsynaptic partners at the target area. Postsynaptic partners then recognize the presynaptic axons to make ensembles, thus establishing a topographic map along the anterior/posterior axis.