NONINVASIVE STUDIES OF CONIFER ROOTS - NUCLEAR-MAGNETIC-RESONANCE (NMR) IMAGING OF DOUGLAS-FIR SEEDLINGS

NONINVASIVE STUDIES OF CONIFER ROOTS - NUCLEAR-MAGNETIC-RESONANCE (NMR) IMAGING OF DOUGLAS-FIR SEEDLINGS
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DOI:
10.1139/x91-217
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发表时间:
1991-11-01
期刊:
CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE
影响因子:
--
通讯作者:
PERRY, D
PERRY, D
中科院分区:
其他
文献类型:
--
作者:
BROWN, DP;PRATUM, TK;PERRY, D

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研制了一种适合于磁共振成像的人造土壤混合物。这种土壤混合物由石英砂、泥炭苔藓和高岭石粘土(体积比为5:3:2)组成,具有自然的质地和一致性,在含水率高达15%的土壤介质中产生高对比度的图像。Douglas-fir(Pseudotsuga menziesii(MIRB.)在直径12.5厘米的容器中,在这种土壤混合物中种植幼苗,并在12个月内定期成像。这些图像显示了根在土壤混合物中不受干扰地生长,并形成了大量的侧根。根据这些图像,计算了根的长度和生长速度。计算的牙根长度与实际挖掘的牙根长度基本一致。未受干扰的侧根的生长速度约为1毫米/天。达到容器壁,然后沿着容器壁向下生长的根的生长速度明显更快,超过2 mm/天。这一现象可能对来自根窗和微型加速器实验的生长速率数据具有重要的意义。
An artificial soil mix suitable for magnetic resonance imaging was developed. This soil mix, consisting of quartz sand, peat moss, and kaolinite clay (5:3:2, by volume), has a natural texture and consistence and produces high-contrast images in soil media with up to 15% water content. Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings were grown in this soil mix in 12.5 cm diameter containers and periodically imaged over a 12-month period. These images showed roots growing undisturbed in the soil mix and initiation of numerous lateral roots. From these images, root lengths and growth rates were calculated. Calculated root lengths were in close agreement with measured lengths of excavated roots. Undisturbed lateral roots had measured growth rates of about 1 mm/day. Roots that reached the container wall and then grew down along the wall had significantly higher growth rates, over 2 mm/day. This phenomenon may have important implications for growth rate data from root window and minirhizotron experiments.