IN-VIVO EFFECT OF 17-BETA-ESTRADIOL ON INTESTINAL CALCIUM-ABSORPTION IN RATS
IN-VIVO EFFECT OF 17-BETA-ESTRADIOL ON INTESTINAL CALCIUM-ABSORPTION IN RATS
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DOI：
10.1016/s0169-6009(08)80062-1
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发表时间：
1994-08-01
期刊：
影响因子：
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通讯作者：
KALU, DN
中科院分区：
文献类型：
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作者：
ARJMANDI, BH;HOLLIS, BW;KALU, DN
以前我们报道，肠细胞含有雌激素受体，17 β-雌二醇在体外增强这些细胞的钙摄取。目前的研究是为了检查17 β-雌二醇对钙和磷的肠道吸收的体内影响。研究了三组大鼠。第1组接受溶剂溶媒。第2组和第3组分别接受5 μ g和40 μ g 17 β-雌二醇/kg体重/天，持续21天。皮下注射激素和溶剂溶媒。在研究期间，大鼠被喂食含0.4%Ca、0.3%P和3.0U维生素D/g的Teklad饮食。在第15-19天的5天时间内评估钙和磷的肠吸收。将胭脂红（25 mg/100 g饲料）添加到大鼠饲料中，以标记粪便收集的开始和结束。给予17 β-雌二醇可增加肠道对钙和磷的吸收。仅钙和接受高剂量17 β-雌二醇的动物的增加显著（P < 0.05）。血清钙和磷水平显着高于17 β-雌二醇治疗组比对照组动物。17 β-雌二醇剂量依赖性地增加尿钙、磷排泄量，高剂量组尿钙、磷排泄量显著增加（P < 0.05）。相比之下，17 β-雌二醇治疗没有显著改变血清甲状旁腺激素和1，25（OH）（2）维生素D的水平。这些结果表明，雌激素的管理，促进体内钙的肠道吸收。尽管血清1，25（OH）（2）维生素D水平不变，但钙吸收增强表明雌激素主要通过增加1，25（OH）（2）维生素D的循环水平来促进钙吸收。
Previously we reported that intestinal cells contain estrogen receptors, and that 17 beta-estradiol enhanced calcium uptake by these cells in vitro. The current study was undertaken to examine the in vivo effects of 17 beta-estradiol on intestinal absorption of calcium and phosphorus. Three groups of rats were studied. Group 1 received solvent vehicle. Groups 2 and 3 received 5 mu g and 40 mu g 17 beta-estradiol/kg body weight/day, respectively, for 21 days. Hormone and solvent vehicle injections were given subcutaneously. Rats were fed a Teklad diet containing 0.4% Ca, 0.3% P and 3.0 U vitamin D/g during the study. Intestinal absorption of calcium and phosphorus was assessed over a 5-day period from day 15-19. Carmine red (25 mg/100 g diet) was added to the rat feed to mark the beginning and end of fecal collections. Administration of 17 beta-estradiol caused an increase in intestinal absorption of calcium and phosphorus. The increase was significant only for calcium, and in the animals that received high-dose 17 beta-estradiol (P < 0.05). Serum calcium and phosphorus levels were significantly greater in 17 beta-estradiol treated than in control animals. The urinary excretion of calcium and phosphorus was also increased in a dose-dependent manner by 17 beta-estradiol, and was significant for both calcium and phosphorus in animals that received high-dose 17 beta-estradiol (P < 0.05). In contrast, 17 beta-estradiol treatment did not significantly alter the serum levels of parathyroid hormone and 1,25(OH)(2)vitamin D. These findings indicate that estrogen administration promotes intestinal absorption of calcium in vivo. The enhanced calcium absorption, in spite of unaltered serum 1,25(OH)(2)vitamin D levels, suggests that estrogen does not promote calcium absorption mainly by increasing the circulating levels of 1,25(OH)(2)vitamin D.