Cadherin-11 is expressed in detrusor smooth muscle cells and myofibroblasts of normal human bladder
Cadherin-11 is expressed in detrusor smooth muscle cells and myofibroblasts of normal human bladder
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DOI：
10.1016/j.eururo.2007.01.052
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发表时间：
2007-10-01
期刊：
影响因子：
23.4
通讯作者：
Schalken, Jack A.
中科院分区：
文献类型：
--
作者：
Kuijpers, Kamiel A. J.;Heesakkers, John P. F. A.;Schalken, Jack A.
目的：近年来发现逼尿肌平滑肌细胞和肌成纤维细胞通过缝隙连接相互连接。然而，缝隙连接不能解释细胞间强的物理相互作用，这促使了对细胞间粘附分子的研究。钙粘蛋白-11是这种分子的候选者，因为它介导收缩性伤口肉芽组织中真皮肌成纤维细胞的相互作用。因此，我们假设逼尿肌平滑肌细胞和肌成纤维细胞之间的物理粘附是由钙粘蛋白-11介导的。本研究的目的是为了测试这一hypothes.Methods：膀胱活检从8个根治性膀胱癌标本速冻，切片，并染色的E-钙粘蛋白，钙粘蛋白-11; α-连环蛋白; β-连环蛋白; γ-连环蛋白;和平滑肌细胞/肌成纤维细胞标记连接蛋白-43，vimentin，结蛋白，平滑肌肌动蛋白，和smoothelin。结果：除E-cadherin外，所有粘附复合物分子在逼尿肌上皮下组织均呈膜特异性阳性表达。所有的活检显示了类似的点状模式表达的钙粘蛋白-11内束平滑肌细胞和亚上皮层的细胞。钙粘蛋白-11是专门位于细胞膜上，在不同的线性domains.Conclusions：据我们所知，这是第一次的证据已经提供了钙粘蛋白介导的平滑肌和上皮下肌成纤维细胞在人膀胱细胞间的相互作用。钙粘蛋白-11很可能在逼尿肌平滑肌细胞和肌成纤维细胞的细胞间物理偶联中起重要作用。(c)2007年欧洲泌尿外科协会。Elsevier B. V.出版，保留所有权利。
Objectives: It has recently been found that detrusor smooth muscle cells and myofibroblasts are coupled via gap junctions. However, gap junctions cannot account for strong physical interaction between cells, which has prompted the search for intercellular adhesion molecules. Cadherin-11 is a candidate for such a molecule, since it mediates the interaction of dermal myofibroblasts in contractile wound granulation tissue. We therefore hypothesised that the physical adhesion between detrusor smooth muscle cells and myofibroblasts is mediated by cadherin-11. The aim of this study was to test this hypothesis.Methods: Bladder biopsies from eight radical cystectomy specimens were snap-frozen, sectioned, and stained for E-cadherin; cadherin-11; (alpha-catenin; beta-catenin; gamma-catenin; and smooth muscle cell/myofibroblast markers connexin-43, vimentin, desmin, smooth muscle actin, and smoothelin. Specimens were analysed by using binocular epifluorescent and confocal laser-scanning microscopy.Results: Specific positive membranous expression of all adhesion complex molecules except E-cadherin was detected in detrusor suburothelial tissue. All biopsies showed a similar punctate pattern of expression for cadherin-11 within bundles of smooth muscle cells and a suburothelial layer of cells. Cadherin-11 was specifically located at the cell membrane, in distinct linear domains.Conclusions: To our knowledge this is the first time evidence has been provided for cadherin-mediated smooth muscle and suburothelial myofibroblast cell-cell interaction in the human bladder. Cadherin-11 most probably plays an important role in the intercellular physical coupling of detrusor smooth muscle cells and also of myofibroblasts. (c) 2007 European Association of Urology. Published by Elsevier B.V. All rights reserved.