Development of H2O2 image sensor for GABA and glutamate imaging
Development of H2O2 image sensor for GABA and glutamate imaging
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开发用于 GABA 和谷氨酸成像的 H2O2 图像传感器
DOI：
10.1109/transducers.2017.7994356
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
K. Sawada
中科院分区：
文献类型：
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作者：
Y. Okumura;S. Mizutani;K. Okumura;T. Iwata;K. Takahashi;K. Sawada
我们成功开发了128×128像素、空间分辨率为23.55μm的H<inf>2</inf>O<inf>2</inf>图像传感器。该传感器由沉积在高灵敏度电位图像传感器上的六氰基铁酸盐(II)、六氰基铁酸盐(III)、酶和金薄膜组成，其中六氰基铁酸盐(II)、六氰基铁酸盐(III)和酶固定在金膜上。 H<inf>2</inf>O<inf>2</inf>图像传感器测量由H<inf>2</inf>O<inf>2</inf>涉及的氧化还原反应引起的溶液和Au膜之间的界面电位，因此传感器信号不受溶液缓冲作用的影响。产量随混合物中Fe<sup>2+</sup>、Fe<sup>3+</sup>的比例呈线性增加；由此证实该传感器可以定性测量氧化还原电位。此外，我们证明该传感器可以可视化 H<inf>2</inf>O<inf>2</inf> 和谷氨酸的扩散行为。这些结果还表明，通过利用产生 H<inf>2</inf>O<inf>2</inf> 的酶反应，可以对血清素、多巴胺等神经递质进行成像。
We have developed H<inf>2</inf>O<inf>2</inf> image sensors of 128×128 pixels with spatial resolution of 23.55 μm successfully. This sensor consisted of hexacyanoferrate(II), hexacyanoferrate(III), enzyme, and an Au thin film deposited on a highly sensitive potential image sensor, where hexacyanoferrate(II), hexacyanoferrate(III), and enzyme were immobilized on the Au film. The H<inf>2</inf>O<inf>2</inf> image sensor measures the interfacial potential between the solution and the Au film caused by a redox reaction involving H<inf>2</inf>O<inf>2</inf>, and thus the sensor signal is not affected by the buffering action of the solution. The output increased linearly with the ratio of Fe<sup>2+</sup>, Fe<sup>3+</sup> of their mixture; thus it was confirmed that the sensor can measure the redox potential qualitatively. Furthermore, we demonstrated that the sensor can visualize the diffusion behavior of H<inf>2</inf>O<inf>2</inf> and glutamate. These results also suggest the possibility of imaging neurotransmitters such as Serotonin, Dopamine etc, by utilizing the enzyme reaction that generates H<inf>2</inf>O<inf>2</inf>.