Calcium ionophore, A23187, induces commitment to differentiation but inhibits the subsequent expression of erythroid genes in murine erythroleukemia cells
Calcium ionophore, A23187, induces commitment to differentiation but inhibits the subsequent expression of erythroid genes in murine erythroleukemia cells
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钙离子载体 A23187 诱导分化，但抑制小鼠红白血病细胞中红系基因的后续表达
DOI：
10.1182/blood.v77.6.1362.1362
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发表时间：
1991
期刊：
影响因子：
20.3
通讯作者：
D. Housman
中科院分区：
文献类型：
--
作者：
J. Hensold;G. Dubyak;D. Housman
小鼠红细胞白血病（MEL）细胞是研究红细胞分化调控过程的有用模型，因为这些细胞暴露于各种化学诱导剂会导致红细胞特异性基因的表达，从而导致细胞不朽性的丧失。此前有研究表明，钙离子载体A23187对导致这些细胞分化的早期细胞事件有影响，但其本身并不足以诱导分化。我们在这里证明，A23187，以及另一种钙离子载体，离子霉素，能够诱导承诺分化。与其他诱导剂不同，持续暴露于A23187可抑制红系特异性基因，β -珠蛋白和Band 3的转录。这种效应不是由于胞质钙浓度的增加，因为离子霉素诱导的细胞产生正常数量的血红蛋白。A23187对MEL细胞的这些影响证实，分化承诺与随后红系基因的转录激活是一个不同的事件。这两种离子载体诱导分化承诺的能力表明，细胞质钙的增加可以触发分化承诺。这些药物在研究负责分化的细胞过程中应该是有用的。
Murine erythroleukemia (MEL) cells are a useful model for studying the processes that regulate erythroid differentiation because exposure of these cells to a variety of chemical inducing agents results in expression of erythroid-specific genes and the resultant loss of cellular immortality. Previously it has been suggested that the calcium ionophore, A23187, has effects on the early cellular events that lead to the commitment of these cells to differentiation, but was not in itself sufficient to induce differentiation. We demonstrate here that A23187, as well as another calcium ionophore, ionomycin, are capable of inducing commitment to differentiation. Unlike other inducing agents, continual exposure to A23187 inhibits transcription of the erythroid- specific genes, beta-globin and Band 3. This effect is not attributable to an increase in cytosolic calcium concentration, because cells induced by ionomycin produce normal amounts of hemoglobin. These effects of A23187 on MEL cells confirm that commitment to differentiation is a distinct event from the subsequent transcriptional activation of erythroid genes. The ability of both ionophores to induce commitment to differentiation suggests that an increase in cytosolic calcium can trigger commitment to differentiation. These agents should prove useful in investigating the cellular processes that are responsible for commitment to differentiation.
DOI：
10.1016/s0021-9258(17)44608-4
发表时间：
1983-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Spiegelman;M. Frank;H. Green
通讯作者：
B. Spiegelman;M. Frank;H. Green
DOI：
10.1073/pnas.78.8.4922
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LEE, AS;DELEGEANE, A;SCHARFF, D
通讯作者：
SCHARFF, D