Nanophase-separated Amphiphilic conetworks as versatile matrixes for optical chemical and biochemical sensors
Nanophase-separated Amphiphilic conetworks as versatile matrixes for optical chemical and biochemical sensors
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DOI：
10.1021/ac060634
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发表时间：
2006-09-15
影响因子：
7.4
通讯作者：
Heinze, Juergen
中科院分区：
文献类型：
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作者：
Hanko, Michael;Bruns, Nico;Heinze, Juergen
作为一类新型传感器矩阵，纳米相分离的两亲聚合物共网络（APCN）为光学化学和生化传感开辟了新的维度。这些共网络由亲水相-我们使用聚(丙烯酸2-羟乙酯)、聚(丙烯酸2-(二甲基氨基)乙酯)或疏水相-聚(二甲基硅氧烷)的聚阳离子聚(丙烯酸2-(三甲基铵)乙酯)砂组成。传感器可以通过简单的基质浸渍来制备。由于纳米相分离，指示试剂被良好固定的区域和有利地负责分析物扩散传输的区域之间存在空间分离，由此可以交换相反相的这些功能。由于相反相之间存在巨大的界面，指示剂的可及性良好，这使得设计高灵敏度的传感器成为可能。为了展示 APCN 作为矩阵的优势，制备了不同的传感器原型，例如：例如，一种方法是根据其与固定化邻联甲苯胺的反应来测定气态氯，另一种方法是根据固定化的溴酚蓝双阴离子来测定气态酸。作为生化传感领域的一项突破，我们还能够提出一种易于生产、光学透明的生化传感器，用于基于共固定化辣根过氧化物酶和 2,2'-azinobis(3-乙基苯并噻唑啉-6-磺酸盐) 来测定非极性有机介质中的过氧化物。
As a novel class of sensor matrixes, nanophase-separated amphiphilic polymeric conetworks (APCNs) open a new dimension for optical chemical and biochemical sensing. These conetworks consist of a hydrophilic phase-we used poly(2-hydroxyethyl acrylate), poly(2-(dimethylamino)ethylacrylate), or polycationic poly( 2-( trimethylammonium) ethyl acrylate) sand of a hydrophobic phase-poly( dimethylsiloxane). Sensors can be prepared by simple impregnation of the matrix. Due to nanophase separation, there is a spatial separation between areas in which the indicator reagents are well immobilized and areas that advantageously take care of the diffusive transport of the analyte, whereby these functionalities of the contrary phases can be exchanged. Thanks to the huge interface between the contrary phases, the accessibility of the indicator reagents is good, which makes it possible to design sensors with high sensitivity. To demonstrate the advantages of APCNs as matrixes, different prototypes of sensors were prepared, e. g., one to determine gaseous chlorine based on its reaction with immobilized o-tolidine and another to determine vaporous acids based on immobilized bromophenol blue dianions. As a breakthrough in biochemical sensing, we are also able to present an easily producible, optically transparent biochemical sensor to determine peroxides in nonpolar organic medias based on coimmobilized horseradish peroxidase and 2,2'-azino- bis(3-ethylbenzothiazoline-6-sulfonate).