Agrobacterium-Mediated Transformation in Liliaceous Ornamental Plants
Agrobacterium-Mediated Transformation in Liliaceous Ornamental Plants
复制标题
DOI：
10.6090/jarq.36.119
复制
发表时间：
2002-07
影响因子：
0.4
通讯作者：
Sakae Suzuki;M. Nakano
中科院分区：
文献类型：
--
作者：
Sakae Suzuki;M. Nakano
利用农杆菌介导法对台湾百合、百子莲等3种百合科观赏植物进行遗传转化研究。orientalis和Muscari armeniacum。三种不同的A.使用了根癌农杆菌，所有这些农杆菌都含有在T-DNA区携带nptll、hpt和gus-内含子基因的双元载体。李斯特而与台湾相思共培养后，未获得转基因组织或植株。根癌农杆菌，尽管在共培养过程中可以在愈伤组织中检测到GUS基因的瞬时表达。另一方面，两种A.早熟禾属orientalis和M.将共培养的胚性愈伤组织转移到含潮霉素（Hyg）的培养基上后，Hyg r愈伤组织经体细胞胚胎发生发育成完整植株，GUS组织化学检测和PCR分析证实大部分植株为转基因植株。Southern杂交分析表明，两种转基因植物的基因组中均整合了1 ~ 5个拷贝的转基因，但大多数转基因植物的基因组中整合了1 ~ 2个拷贝。建立了农杆菌介导的转化体系。早熟禾属orientalis和M.杏可能是其遗传改良和分子生物学研究的有用工具。
Studies on Agrobacterium-mediated transformation in 3 Liliaceous ornamental plants, Lilium formosanum, Agapanthus praecox ssp. orientalis and Muscari armeniacum, were described. Three different strains of A. tumefaciens were used, all of which harbored the binary vector carrying the nptll, hpt and gus-intron genes in the T-DNA region. For L. formosanum, no transgenic tissues nor plants were obtained after co-cultivation of organogenic calli with A. tumefaciens, although transient expression of the gus gene could be detected in the calli during co-cultivation. On the other hand, several hygromycin-resistant (Hyg r ) cell clusters were obtained for both A. praecox ssp. orientalis and M. armeniacum following the transfer of co-cultivated embryogenic calli onto hygromycin (Hyg)-containing media. Hyg r calli developed into complete plants via somatic embryogenesis, and most of them were confirmed to be transgenic plants based on GUS histochemical assay and PCR analysis. Southern blot analysis revealed the integration of 1 to 5 copies of the transgene into the genome of the transgenic plants of both 2 species, but most of them had 1 or 2 copies. Agrobacterium-mediated transformation systems developed for A. praecox ssp. orientalis and M. armeniacum may be useful as a tool for their genetic improvement as well as molecular biology studies.