Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.
Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.
复制标题
基于 MMTV 的条件逆转录病毒载体包装细胞系的构建和表征。
DOI：
10.1016/0006-291x(89)92236-5
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发表时间：
1989
影响因子：
3.1
通讯作者：
Günzburg,WH
中科院分区：
文献类型：
--
作者：
Salmons,B;Moritz-Legrand,S;Garcha,I;Günzburg,WH
用劳斯肉瘤病毒的LTR取代具有完整生物活性的小鼠乳腺肿瘤病毒（MMTV）前病毒DNA的5′ LTR，构建了嵌合前病毒。在转染到允许细胞中后，该前病毒指导MMTV gag和env结构蛋白的合成，但在编码这些蛋白的RNA的包装中受损。用基于MMTV的载体构建体超转染这些细胞导致以比先前描述的辅助细胞系更高的效率产生感染性重组病毒。这种基于MMTV的逆转录病毒载体系统将允许研究插入基因的条件表达对感染细胞的影响。
A chimeric provirus in which the 5′ LTR of a complete biologically active Mouse Mammary Tumour Virus (MMTV) proviral DNA has been replaced with the Rous Sarcoma Virus LTR has been constructed. Upon transfection into permissive cells, this provirus directs the synthesis of the MMTV gag and env structural proteins, but is impaired in packaging of the RNAs that encode these proteins. Supertransfection of these cells with MMTV based vector constructs results in the production of infectious recombinant virus at a higher efficiency than with previously described helper cell lines. Such a retroviral vector system based on MMTV will allow the study of the effects of conditional expression of inserted genes upon infected cells.